奈米金粒子放大氧化還原電流訊號之單一細菌濃度感測晶片

細菌的快速分離和檢測在臨床醫療診斷、食物中毒、水汙染和生物恐怖攻擊等領域是極為重要的發展議題。傳統細菌檢測需先進行菌株培養數天後，再利用特定的染色或是免疫代謝等方法，來判讀菌種與濃度。針對敗血症而言，最重要的目標在於縮短檢測時間至1～2小時內判定菌種，同時在病人尚未發病或是發病初期的階段就能夠在病人的檢體內（血液、尿液等），直接分離並檢測出細菌的濃度。因此吾人發展－可攜式微流體實驗室晶片進行樣本的前處理與分離，同時利用奈米金粒子放大電化學氧化還原訊號，達到單一細菌檢測的可能性。目前此晶片在45分鐘內可有效的區別3種細菌（K. pneumoniae, S. aureus and P. Aeruginosa），而樣本的最低檢測極限值可達3.3 cell/μL。

關鍵字

奈米金粒子；單一細菌檢測；氧化還原訊號放大

並列摘要

Rapid separation and detection to bacteria are extremely important in the field of clinical diagnostics, food poisoning, water pollution, bioterrorist attacks and other developing issues. The traditional bacterial detection requires carrying out germiculture several days and re-using specific staining or immune metabolism to interpret the strain and concentration. As to sepsis, the most important issue is to shorten detected time to 1~2 hours in deciding strains. At the same time, on the onset of early stage, patient can be diagnosed with samples (blood, urine, etc.) from direct separating and detecting the concentration of the bacteria. In this research, a portable microfluidic chip is used to sample for pretreatment and separation, while taking advantage of the gold nanoparticles to magnify electrochemical redox signals as to reach the possibility for single bacterial detection. As a result, the minimum detection rate of this portable microfluidic chip in 45 minutes among three bacteria samples (K. pneumoniae, S. aureus and P. Aeruginosa) can be effectively differentiated up to 3.3 cell/μL.

並列關鍵字

gold nanoparticle ； single bacteria detection ； redox signal amplification

國際替代計量

奈米金粒子放大氧化還原電流訊號之單一細菌濃度感測晶片

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