液滴式免疫分析晶片

Droplet-based Immunoassay Chip

張耀仁(Yaw-Jen Chang) ； 丁子洹(Zi-Huan Ding)

《先進工程學刊》 7卷4期 (2012/10) Pp. 171-176

https://doi.org/10.29948/JAE.201210.0004

摘要

本研究設計一液滴式免疫分析晶片，利用微流道結構產生液滴，作為微型反應槽，以進行免疫分析試驗。不同試劑經過矽油包覆成液滴後，在微流道中產生液滴融合及混合，最後排列於微流道內，以進行後續培養和螢光檢測。本晶片使用鎳鈷合金電鍍層以抓取並固定具有組氨酸標記之蛋白質，固定機制是基於固定化金屬親和層析法。實驗結果顯示，透過流速的控制，可以產生不同大小的液滴，雖然液滴內所包覆試劑量很少，但是鎳鈷合金鍍層可以有效地與具組氨酸標記之蛋白質產生鍵結，所以在免疫分析試驗後可以得到很好的螢光檢測值。本液滴式免疫分析晶片具有試劑用量少、試劑間無彼此交叉污染、操作自動化、成本低廉等優點。

關鍵字

微液滴 ； 生物素 ； 鎳鈷鍍面 ； 微流道晶片

並列摘要

In this paper, a design of droplet-based immunoassay chip is presented. Droplets formed by using the microfluidic structures act as micro-reactors for carrying out immunoassays. Different reagents are encapsulated by silicon oil to form droplets respectively. Through the droplet fusion and mixing, these fused droplets are aligned in the microchannel for further incubation and fluorescence detection. A thin-film of Ni-Co alloy is electroplated on the surface of this proposed chip for immobilizing histidine-tagged protein, based on the theory of immobilized metal affinity chromatography. Experimental results show that different sizes of droplets can be generated through the control of the flow rate. Although only small amount of reagent is encapsulated in a droplet, strong fluorescence intensity can be detected after the immunoassays when it comes from the effective affinity binding between the Ni-Co film and the histidine-tagged protein. This proves droplet-based immunoassay chip possesses many advantages including small amount of reagent consumption, no cross-contamination between reagents, automatic manipulation, and low cost.

並列關鍵字

micro-droplet ； biotin ； Ni-Co electrodeposited surface ； microfluidic chip