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運動訓練對人體體內自由基產生及抗氧化酵素之影響

The Effect of Free Radical and Antioxidant Enzymes in Human Blood

摘要


In order to elucidate the relationship between ROS homeostasis and physical training, the ROS levels of blood were measured before, during and after a certain physical challenge by the method of chemiluminescence. The intensity of physical challenge is designed to make volunteers reach 85% of maximum heartbeat and sustain till the subject willing to rest. The female volunteers were categorized into three groups. SED Group: as a sedentary subjects group (n=12), STT Group: short term exercise training subjects group (n=11), and LTT Group: long term exercise training group. Our study's results show that the ROS turnovers of these two groups are quite different during physical challenge. The ROS level seems to be consistent in the group of regular exercise, but increasing in the group of seldom exercise during physical challenge. All volunteers were capable to neutralize ROS after a two-hour rest. In spite of insignificant change in some clinical parameters of blood, the activities of some scavenger enzymes such as SOD、catalase、GPx, seem to be decreased in the group of regular exercise. By comparing before with after 6-weeks-aerobic training, the group of seldom exercise showed decreased but quite similar patterns of the ROS level during physical challenge. Even after 6-weeks training, the ROS level measured before, during and after the physical challenge were still higher than the group of regular exercise.

並列摘要


In order to elucidate the relationship between ROS homeostasis and physical training, the ROS levels of blood were measured before, during and after a certain physical challenge by the method of chemiluminescence. The intensity of physical challenge is designed to make volunteers reach 85% of maximum heartbeat and sustain till the subject willing to rest. The female volunteers were categorized into three groups. SED Group: as a sedentary subjects group (n=12), STT Group: short term exercise training subjects group (n=11), and LTT Group: long term exercise training group. Our study's results show that the ROS turnovers of these two groups are quite different during physical challenge. The ROS level seems to be consistent in the group of regular exercise, but increasing in the group of seldom exercise during physical challenge. All volunteers were capable to neutralize ROS after a two-hour rest. In spite of insignificant change in some clinical parameters of blood, the activities of some scavenger enzymes such as SOD、catalase、GPx, seem to be decreased in the group of regular exercise. By comparing before with after 6-weeks-aerobic training, the group of seldom exercise showed decreased but quite similar patterns of the ROS level during physical challenge. Even after 6-weeks training, the ROS level measured before, during and after the physical challenge were still higher than the group of regular exercise.

參考文獻


Alessio, H.M.(1993).Exercise-induced oxidative stress.Medicine and Science in Sport and Exercise.25(2),218-224.
Bejma, J.,Ji, L.L.(1999).Aging and acute exercise enhance free radical generation in rat skeletal muscle.Journal of Applied Physiology.87(1),465-470.
Brites, F.D.,Evelson, P.A.,Christiansen, M.G.,Nicol, M.F.,Basilico, M.J.,Wikinski, R.W.,Llesuy, S.F.(1999).Soccer players under regular training show oxidative stress but an improved plasma antioxidant status.Clinical Science.96,381-385.
Child, R.,Brown, S.,Day, S.,Donnelly, A.,Roper, H.,Saxton, J.(1999).Change in indices of antioxidant status, lipid peroxidation and inflammation in human skeletal muscle after eccentric muscle actions.The Biochemical Society and the Medical Research Society.96,105-115.
Clanton, T.L.,Zuo, L.,Klawitter, P.F.(1999).Oxidants and skeletal muscle function: physiologic and pathophysiologic implications.Society for Experimental Biology and Medicine.222,253-262.

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