The purpose of this study is the design of App teaching material content for mobile learning in printing professional courses. In this study, literature review is the first way to collate and analyze the relevant literature materials to understand the development of mobile learning teaching material content in App, the work flow of production and the teaching material content of printing professional courses requirements. And then in depth interviews, first of all, with the relevant literature materials and the subject matter of the printed education materials to design a systematic printing professional course, and through experts with working or teaching experience to assist in validation of course content. The results show that the printing professional course can provide students with complete course content, but the part of assisting students to improve their knowledge and practical ability can only be applied to major related students.