Pseudomonas cichorii引起之番茄細菌性葉斑病

Occurrence of Bacterial Leaf Spot of Tomato Caused by "Pseudomonas cichorii" in Taiwan

蔡佳欣(Chia-Hsin Tsai) ； 安寶貞(Pao-Jen Ann) ； 呂昀陞(Yun-Sheng Lu) ； 陳美德(Mei-De Chen) ； 黃淑苓(Shu-Ling Hwang) ； 彭玉花(Yu-Hua Peng)

《台灣農業研究》 63卷2期 (2014/06) Pp. 143-150

https://doi.org/10.6156/JTAR/2014.06302.05

摘要

南投縣與花蓮縣部分番茄栽植田於2010-2011年期間，發現植株葉片出現墨綠至暗褐色之不規則形或圓形壞疽之水浸狀斑點病徵，經由病組織分離出一種病原細菌，該細菌可誘導萬國士煙草產生過敏性反應。繼將該細菌接種至番茄葉片，可產生相同病徵，完成柯霍氏法則測試。此病原細菌經生理生化測試、Biolog鑑定系統分析、16S rDNA序列比對，並以專一性引子對SfL1/SfR2進行PCR增幅鑑定，確認此病原細菌為Pseudomonas cichorii。此為P. cichorii感染番茄葉片在台灣的首次報導。以濾紙圓盤擴散法測試10種市售藥劑對該病原菌的抑制圈大小，結果顯示以鏈四環黴素之抑制效果最佳。

關鍵字

番茄 ； 細菌性葉斑病 ； 細菌性葉斑病菌 ； 藥劑篩選

並列摘要

During 2010-2011 an unknown disease of tomato was found in Nantou and Hualien counties. Dark green to dark brown irregular or round spots were observed on the diseased leaves. The bacteria isolated from the infected tissues can induce tobacco hypersensitive reaction and their pathogenicity was verified by Koch’s postulates. The pathogen was further identified as ＂Pseudomonas cichorii＂ based on physiological and biochemical tests, Biolog identification, 16S rDNA sequence analysis, and specific primers Sf1/SfR2 following polymerase chain reaction. This is the first report of ＂P. cichorii＂-elicited tomato bacterial leaf spot disease in Taiwan. Among the tested agrochemicals, the mixture of streptomycin and tetracycline has the highest efficacy in inhibiting bacterial growth.

並列關鍵字

Tomato ； Bacterial leaf spot ； "Pseudomonas cichorii" ； Agrochemical screening