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豬萎縮性鼻炎巴氏桿菌類毒素菌苗之研發及其效力試驗

Protection Against Swine Atrophic Rhinitis by Vaccination with Pasteurella Multocida Toxoid Bacterin

摘要


By combining PMTcd toxoid with inactivated B. bronchiseptica and P. multocida type A, an experimental atrophic rhinitis (AR) bacterin-toxoid was developed in this study. Immunologic tests were carried out to assess its efficacy. Mice and guinea pigs vaccinated with the AR bacterin-toxoid were proved to have excellent protection after challenged with PMTcd. The piglets born by the vaccinated pregnant sows were shown with high maternal antibody and good protection levels. The average maternal antibody titers in 3 days old and 1 week-old piglets were measured and shown to have successful protection by challenged with PMTcd at 1 week old without showing AR clinical signs and lesions. Prior to and up to 1 month after challenge, the average weight gain of the experimental piglets showed significant differences (p<0.05) from the positive control ones. Another protection test was also conducted in our studies. Sows and their offspring were divided into 5 groups as the groups A (sows immunized and piglets vaccinated twice at 1 and 3 weeks of age), B (sows immunized but piglets vaccinated only once at 1 week old), C (sows immunized but piglets without vaccinated), D (sows not immunized but piglets vaccinated twice at 1 and 3 weeks of age), and positive control group (sows and piglets without immunized). AII the piglets of these 5 groups were challenged with PMTcd at 5 weeks of age. The results showed that group A and D piglets had good antibody response and excellent protection against toxin challenge. Group B and C piglets only possessed uncomplete protection. After challenged, the lesions of AR were evidently seen in some piglets. The piglets from control group showed marked clinical signs and lesions of nasal atrophy after challenge. To compare the weight gains among these experimental and control piglets challenged with PMTcd after 1 month and 2 months, the results showed that the piglets of group A had best growth performance, followed by group D, group B, group C and control group. The weight gain between experimental groups and control group showed significant differences (p < 0.05). Another significant differences between groups A, D and groups B, C (p < 0.05) were also noticed.

並列摘要


By combining PMTcd toxoid with inactivated B. bronchiseptica and P. multocida type A, an experimental atrophic rhinitis (AR) bacterin-toxoid was developed in this study. Immunologic tests were carried out to assess its efficacy. Mice and guinea pigs vaccinated with the AR bacterin-toxoid were proved to have excellent protection after challenged with PMTcd. The piglets born by the vaccinated pregnant sows were shown with high maternal antibody and good protection levels. The average maternal antibody titers in 3 days old and 1 week-old piglets were measured and shown to have successful protection by challenged with PMTcd at 1 week old without showing AR clinical signs and lesions. Prior to and up to 1 month after challenge, the average weight gain of the experimental piglets showed significant differences (p<0.05) from the positive control ones. Another protection test was also conducted in our studies. Sows and their offspring were divided into 5 groups as the groups A (sows immunized and piglets vaccinated twice at 1 and 3 weeks of age), B (sows immunized but piglets vaccinated only once at 1 week old), C (sows immunized but piglets without vaccinated), D (sows not immunized but piglets vaccinated twice at 1 and 3 weeks of age), and positive control group (sows and piglets without immunized). AII the piglets of these 5 groups were challenged with PMTcd at 5 weeks of age. The results showed that group A and D piglets had good antibody response and excellent protection against toxin challenge. Group B and C piglets only possessed uncomplete protection. After challenged, the lesions of AR were evidently seen in some piglets. The piglets from control group showed marked clinical signs and lesions of nasal atrophy after challenge. To compare the weight gains among these experimental and control piglets challenged with PMTcd after 1 month and 2 months, the results showed that the piglets of group A had best growth performance, followed by group D, group B, group C and control group. The weight gain between experimental groups and control group showed significant differences (p < 0.05). Another significant differences between groups A, D and groups B, C (p < 0.05) were also noticed.

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