Case Report: Identification of Pathogenic Porcine Circovirus in Pigs with Post-Weaning Multisystemic Wasting Syndrome in Taiwan

病例報告：本省豬離乳後多系統性消耗症環狀病毒之確認

陳世平(Shih-Ping Chen) ； 翁有助(Yeou-Juh Weng) ； 蔡敬屏(Ching-Ping Tsai) ； 張文發(Woon-Fa Chang) ； 黎南榮(Nan-Jung Li) ； 蔡清恩(Chin-En Tsai) ； 邱明堂(Ming-Tang Chiou)

《中華民國獸醫學會雜誌》 27卷4期 (2001/12) Pp. 251-255

https://doi.org/10.7009/JTCSVS.200112.0251

摘要

台灣中部地區一場飼有660頭母頭的一貫場於1999年爆發離乳後多系統性消耗症(Post-weaning multisystemic wasting syndrome; PMWS)，本症好發於5-10週齡的保育豬及肥育前期豬隻，病豬呈現持續性的消瘦及呼吸困難。組織病理學檢查主要病變為支氣管性間質性肺炎及肉芽腫性淋巴腺病，淋巴結內有巨噬球的浸潤，其內可見獨持的多個大的嗜鹼性“葡萄樣”細胞質內包涵體。於腸繫膜淋巴結研磨的乳劑及超薄切片中均可見環狀病毒樣的病毒顆粒。以聚合酵素鏈鎖反應(polymerase chain reaction; PCR)可從淋巴結、肺臟、脾臟及扁桃腺中檢出環狀病毒(porcine circovirus; PCV)的核酸片段。本病例綜合臨床症狀、病理變化、電子顯微鏡檢查及PCR檢測結果，確認PMWS已存在台灣的養豬場中。

關鍵字

離乳後多系統性消耗症 ； 環狀病毒 ； 聚合酵素鏈鎖反應 ； 豬

並列摘要

An outbreak of post-weaning multisystemic wasting syndrome (PMWS) characterized by progressive weight loss and dyspnea was observed in weaning pigs at a 660-sow continuous- flow operation in central Taiwan in 1999. The disease was observed mainly in weaning and growing pigs. The histopathologic findings in the PMWS pigs were bronchointerstitial pneumonia and granulomatous lymphoadenitis, with unique large, multiple, basophilic ＂botryoid＂ intracytoplasmic inclusions forming within the histiocytes of the lymph node. These findings resemble those in pigs with PMWS recently reported in North America and Europe. Ultrastructurally, porcine circovirus (PCV)-like particles were observed in mesenteric lymph nodes from the ultra-thin sections and homogenates preparations using transmission and scanning electron microscopy respectively. PCV nucleotides were also amplified by a polymerase chain react ion (PCR) from the lymph nodes, lungs, spleen, and tonsils. This study verifies that outbreaks of PMWS have occurred in Taiwan.

並列關鍵字

PMWS ； Circovirus ； PCR ； Porcine