台灣地區牛羊副結核病及山羊藍舌病之抗體調查

Serological Survey on the Antibodies Against Paratuberculosis in Cows and Goats and Bluetongue Disease in Goats in Taiwan

林郁婷(Yu-Ting Lin) ； 潘漢恩(Han-En Pan) ； 蔡向榮(Hsiang-Jung Tsai)

《中華民國獸醫學會雜誌》 27卷4期 (2001/12) Pp. 256-261

https://doi.org/10.7009/JTCSVS.200112.0256

摘要

以市售牛用酵素結合免疫吸附法(enzyme-linked immunosorbent assay; ELISA)套組檢測87年度台灣地區七個縣份牛隻副結核病抗體存在情形，結果牛場抗體陽性率為50% (63/126)，牛隻抗體陽性率為6.87% (132/1921)。以同一套組及瓊脂凝膠免疫擴散法(agar gel immunodiffusion; AGID)同時檢測310頭山羊血清，兩者之結果完全不具相關性(kappa=-0.08)，亦即牛用ELISA套組不適用於山羊之抗體檢測。經以AGID檢測台灣地區八個縣市山羊副結核病抗體，結果山羊場抗體陽性率為9.02%(11/122)，羊隻抗體陽性率為1.98%(36/1815)，此為台灣地區首次羊隻副結核病抗體調查報告。另外以AGID調查上述羊隻血清中藍舌病抗體存在情形，結果皆呈陰性，證實台灣山羊可能無藍舌病之存在。

關鍵字

副結核病 ； 藍舌病 ； 酵素結合免疫吸附法 ； 瓊脂凝膠免疫擴散法

並列摘要

A commercial enzyme-linked immunosorbent assay (ELISA) kit for the detection of antibodies against paratuberculosis in cattle was used to examine cattle serum samples collected in 1998-1999. The result indicted that the dairy herd positive rate was 50% (63/126) and the dairy cattle positive rate was 6.87% (132/1921). Total of 310 goat serum samples were tested both by the same ELISA kit and an agar gel immunodiffusion test (AGID), the results obtained by the two tests was totally unrelated to each other (kappa=-0.08). The AGID test was used to examined antibodies against paratuberculosis in goat serum samples collected in 1998-1999. The results revealed a herd positive rate of 9.02% (11/122) and a goat positive rate of 1.98% (36/1815). All the goat serum samples were all showed a negative reaction when they were examined for the presence of the bluetongue disease antibody by AGID. Thus it is suggested that bluetongue disease may not present in goat population in Taiwan.

並列關鍵字

Paratuberculosis ； Bluetongue disease ； Enzyme-linked immunosorbent assay ； ELISA ； Agar gel immunodiffusion ； AGID