台灣犬幼犬血清蛋白質及其電泳之輪廓

The Serum Protein and Its Electrophoretic Profile of Young Formosan Dogs

莊士德(Shih-Te Chuang) ； 馮翰鵬(Hang-Poung Fung) ； 張力天(Cicero Lee-Tian Chang) ； 賴富健(Fu-Jiann Lai) ； 劉邦成(Pan-Chen Liu)

《中華民國獸醫學會雜誌》 27卷4期 (2001/12) Pp. 262-272

https://doi.org/10.7009/JTCSVS.200112.0262

摘要

本研究之目的乃在了解台灣犬幼犬之血清蛋白質及其電泳之輪廓的變化。幼犬在28週研究期間，其血清總蛋白質濃度皆比成犬低，在統計學上呈顯著性差異(P＜0.05)至極顯著性差異(P＜0.001)。利用血清蛋白質電泳圖經密度分析儀分析結果，單隻成犬白蛋白帶(44.6%)略高於第8週齡、單隻幼犬(43.7%)；成犬除了α_1球蛋白帶(14.3%)比幼犬(22.4%)低外，其餘的α_1、β_1、β_2及γ球蛋白帶皆比幼犬高。以蛋白質電泳分析各種血清蛋白質濃度，在研究期間可見幼犬白蛋白濃度皆比成犬低，呈顯著性差異(P＜0.05)至極顯著性差異(P＜0.001)。幼犬的α_1、α_2及β_1球蛋白濃度皆較成犬高，且均呈顯著性差異(P＜0.05)至極顯著性差異(P＜0.001)。幼犬β_1球蛋白濃度較成犬低，其變化無規則性，呈顯著性差異(P＜0.05)至極顯著性差異(P＜0.001)。幼犬β_2球蛋白濃度明顯較成犬低，除喝初乳後1日齡外，其餘皆呈極顯著性差異(P＜0.001)。幼犬在剛出生未喝初乳時，無γ球蛋白濃度，喝過初乳後1日齡γ球蛋白濃度會顯著上升，之後快速下降，於3週齡後會隨年齡成長而緩慢上升。幼犬的γ球蛋白濃度與成犬相較，呈顯著性差異(P＜0.05)至極顯著性差異(P＜0.001)。

關鍵字

台灣犬 ； 血清蛋白質 ； 電泳

並列摘要

The main purpose of this study is to realize the serum protein and its electrophoretic profile of young Formosan dogs ranging from birth to 28-week-olds. The total serum protein concentrations for young dogs were lower than adults (P＜0.05 to P＜0.001). The electrophoretic analysis of serum protein bands using density analyzer showed that albumin band (43.7%) of an eight-week-old dog was lower than an adult one (44.6%). The _α1 globulin band (22.4%) for the young dog was higher than the band (14.3%) for the adult, but the other α_2-, β_1-, β_2- and γ-globulin bands for the young dog was lower than the bands for the adult. The result of the electrophoretic analysis of serum protein concentrations showed that the young dogs contained lower albumin concentrations than the adults (P＜0.05 to P＜0.001). The α_1- and α_2--globulin concentrations for young dogs were higher than the concentrations for the adults (P＜0.05 to P＜0.001). The β_1- globulin concentrations for the young dogs were lower than the concentrations for the adults, and the variations were irregular (P＜0.05 to P＜0.001). The β_2--globulin concentrations for young dogs were obviously lower than the concentrations for the adults (P＜0.001). The 1-day-old puppies before colostrum intake had no γ-globulin. However, after colostrum intake, the γ-globulin concentrations in the puppies obviously increased then rapidly decreased. In young dogs of 3 weeks or older, the γ-globulin concentrations slowly increased with age. The γ-globulin concentrations in young dogs were lower than the concentrations in the adults (P＜0.05 to P＜0.001).

並列關鍵字

Formosan dog ； Serum protein ； Electrophoresis