以組織病理學及聚合酶鏈反應證實犬瘟熱病毒及腺病毒雙重感染

The Detection of Dual Infection of Canine Distemper Virus and Canine Adenovirus by Histopathology and Polymerase Chain Reaction

游忠霖(Chung-Lin Yu) ； 闕玲玲(Ling-Ling Chueh) ； 陳秀瓊(Hsiu-Chiung Chen) ； 劉振軒(Chen-Hsuan Liu)

《中華民國獸醫學會雜誌》 27卷4期 (2001/12) Pp. 288-294

https://doi.org/10.7009/JTCSVS.200112.0288

摘要

本篇論文旨在報告兩隻幼犬雙重感染犬瘟熱病毒及腺病毒。患犬一為3月齡混種母犬，另一為7月齡混種公犬，均未有施打疫苗記錄。臨床上共同症狀為發燒、嘔吐、下痢、嚴重眼鼻分泌物、呼吸急促及神經症狀。死亡後進行病理解剖檢查，兩犬隻肉眼病變均以肺臟潮紅及觸摸較堅實為主，組織病理學特徵為壞死性支氣管炎及瀰漫性間質性肺炎；支氣管脫落上皮細胞及肺泡內巨噬細胞出現犬瘟熱病毒(canine distemper virus)嗜酸性核內包涵體及犬腺病毒(canine adenovirus)嗜鹼性核內包涵體，上述兩種包涵體亦出現在胃、小腸及睪丸，但並未出現於肝臟。使用反轉錄聚合酶鏈反應(RT-PCR)，兩隻犬的腦、肺及淋巴組織皆可檢測出犬瘟熱病毒的P基因核酸429 bp片段，而使用聚合酶鏈反應(PCR)，兩隻犬的肺亦檢測出犬腺病毒的pVⅡ基因核酸226 bp片段。綜合上述病理學變化及核酸診斷結果，證實此二犬隻同時罹患犬瘟熱病毒及犬腺病毒感染。因此，臨床上治犬瘟熱時，其他病毒合併感染的可能性亦不容忽視。

關鍵字

犬瘟熱病毒 ； 犬腺病毒 ； 雙重感染

並列摘要

This is a report of a dual infections of canine distepmer virus (CDV) and canine adenovirus (CAV) in two young dogs. The common clinical signs were fever, vomiting, diarrhea, severe ocular and nasal discharge, tachypnoea and nervous sign. Grossly, both lungs in two dogs were reddish and consolidated. The characteristic microscopic lesions were necrotizing bronchiolitis and diffuse bronchointerstitial pneumonia associated with eosinophilic and basophilic intranuclear inclusion bodies resembling CDV and CAV infections respectively in the bronchiolar epithelial cells and alveoli. Both CDV and CAV inclusion bodies were variably observed in the stomach, small intestine and testis, but not liver. With RT-PCR, CDV was detected from the brain, lungs, and lymphoid system. Moreover, PCR was successfully employed to detect CAV from the lungs. Combination of pathological examination and polymerase chain reaction (PCR) provides a more sensitive and specific method to confirm dual viral infection.

並列關鍵字

Canine distemper virus ； Canine adenovirus ； Dual infection