Cephalexin於豬之殘留性試驗

本研究旨在探討Cephalexin monohydrate於豬之適宜口服劑量及投藥後於組織中之殘留分析。先由病豬所分離之不同病原菌共81株進行Cephalexin monohydrate 之MIC (Minimal inhibitory concentration )值測定，結果：以Staph ylococcus spp.及Escherichia coli敏感性較佳，MIC_90為14μg /mL。故以相當於MIC目值之2及3倍(即28及42 mg /kg體重)兩種劑量；分別口服(加於飼料)一次投藥於3頭豬，結果28及42mg /kg劑量組於投藥後2小時血清中達最高峰(分別為13.1及19.5月/mL)，且兩劑量組皆未造成豬隻之肝腎急性病變，故選擇42 mg /kg為最適宜之口服劑量。另取肉豬20頭，每頭每天於飼料中口服投與Cephalexin monohydrate一次(劑量42mg/kg體重)，共5天。停藥後第1、3、5…及20天等10個時段各屠宰2頭，取各組織進行殘留量H P LC分析。結果：停藥後肝、腎及血清於第一天即檢測不到(即低於0.25 ppm)；肌肉中於第一天有0.18 ppm殘留，第九天則檢測不到(即低於0.05 ppm)。綜合以上結果顯示，Cephalexin monohydrate口服投藥給予豬隻之停藥期直為14天。

關鍵字

雪華力新；豬；口服劑量；殘留；高效液相層析法

並列摘要

This study attempts to determine not only an appropriate dosage of Cephalexin monohydrate, but also Cephalexin monohydrate residue in swine tissue after the drug is administered orally. The in vitro antibacterial activity of cephalexin against 4 varieties of clinical bacterial isolates of a total of 81 strains from swine were evaluated. Among the bacteria tested, Staphylococcus spp. and, Escherichia coli are the most susceptible to cephalexin and the drug inhibits more than 90% of the strains (MIC90) at a concentration of 14 μg/mL. Two doses (28 or 42 mg/kg body weight) of Cephalexin monohydrate were administered orally, respectively, to three pigs. Experimental results indicate that the peak serum concentrations of Cephalexin monohydrate appeared two hours after the amounts of drug administered were 13.1 and 19.5 μg/mL respectively. Additionally, no toxicity was found in the liver and kidney with both two dosages and, therefore, the proper dose of oral administration is 42 mg/kg bodyweight. Cephalexin monohydrate was administered orally in feed at a dose of 42 mg/kg bodyweight daily for consecutive 5 days to twenty pigs. The residues of Cephalexin monohydrate in flesh, serum, liver and kidney of two pigs from each group including control were then determined by HPLC at 1, 3, 5... and 20 days (total of 10 intervals) after withdrawing the drug. Moreover, no cephalexin (above 0.25 ppm) was detected accordingly in the serum, kidney, and liver of various time intervals after withdrawing the drug. Whereas, the muscle residual level of 0.18 ppm appeared on the first day until the seventh day and no cephalexin (above 0.05 ppm) was detected on the ninth day. Our results further demonstrate that the withdrawal time of Cephalexin monohydrate via oral administration in swine is 14 days.

並列關鍵字

Cephalexin ； Swine ； Oral dosage ； Residue ； High performance liquid chromatography(HPLC)

國際替代計量

Cephalexin於豬之殘留性試驗

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