Effects of Dietary Lemon Essential Oils on Growth, Biochemical Parameters, Nutrient Digestibility, and Histology of Hepatocyte and Intestinal Villi of Grouper, Epinephelus coioides

Li-Chao Zhuo ； Yu-Hung Lin

《臺灣水產學會刊》 48卷1期 (2021/03) Pp. 1-10

https://doi.org/10.29822/JFST.202103_48(1).0001

摘要

A growth experiment was designed to investigate the effects of dietary supplementation with lemon essential oil (LEO) on growth, biochemical parameters, nutrient digestibility, and histology of hepatocyte and intestinal villi for grouper, Epinephelus coioides. Three diets containing 0 (LEO0), 300 (LEO300) and 600 mg/kg (LEO600) of LEO were randomly delivered to three groups (n=3) of juveniles (initial weight: 18.79±0.48 g) in a recirculation system for eight weeks. Weight gain, feed efficiency, intraperitoneal fat content, survival, hematological parameters, and hepatocyte diameter, intestinal villi area, height, and width exhibited no significant difference (p＞0.05) within the three dietary groups. Activity of plasma lysozyme and hepatic superoxide dismutase (SOD) was higher in fish fed LEO300 and LEO 600 diets than that in the control group. Fish fed the LEO300 diet had higher hepatic malondialdehyde content than other two groups. Dry matter, Fe, and Zn digestibilities were higher in fish fed the LEO0 and LEO300 diets than those in fish fed the LE600 diet. Calcium digestibility was higher in fish fed the LEO300 diet than in the other groups. The results indicated that LEO supplementation in diet can improve the plasma lysozyme and hepatic SOD activity of grouper. No positive effect of LEO was found on growth, nutrient digestibility and histology of hepatocyte and intestinal villi for the fish.

關鍵字

grouper ； lemon essential oils ； immune responses ； nutrient digestibility ； histology