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Effect of different light qualities on the growth and fatty acid composition of Cyclotella meneghiniana (Bacillariophyta)

摘要


Corbicula fluminea is a freshwater shellfish that has great industrial value in Taiwan. The microalga it mainly feeds on is Cyclotella meneghiniana. This study investigated the optimal light conditions for the growth of C. meneghiniana and the effect of light quality on its fatty acid composition. A pre-experiment showed that little to no NH_3-N was consumed by C. meneghiniana in the absence of carbon source. In contrast, NH_3-N concentration decreased greatly when carbon source was provided. Then, this study used light-emitting diode (LED) with blue-ray (430±10 nm), red-ray (670±10 nm), cold white (color temperature: 6000-8000 K), white (color temperature: 3000-6000 K), and warm white (color temperature: 2700-3000 K) at 2000 Lux to culture C. meneghiniana at 25 °C. After 27 days, the blue-ray LED resulted in the highest biomass productivity (7.9×10^3 cells ml^(-1) day^(-1)). The biomass productivities of the other groups, arranged in descending order, were 3.3×10^3, 1.6×10^3, 1.8×10^3 and 1.4×10^3 cells ml^(-1) day^(-1) (red, cold white, white, and warm white, respectively). After analyzing the fatty acid composition of C. meneghiniana with gas chromatography, the results showed that this microalga mainly produced the fatty acids C14:0, C16:0, C16:1, C16:3, C18:0, C18:1 and C20:5. The highest C16:3 fatty acid proportion (12.75 %) was observed in C. meneghiniana cultured under cold white light, and C20:5 (12.76 %) under white light. However, the highest PUFA proportion (25.31 %, total of C16:3 and C20:5 proportions) was observed in cultures under warm white light.

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