Increased Expression of Matrix Metalloproteinase-2 in Oral Cells after Short-Term Stimulation and Long-Term Usage of Areca Quid

短期與長期的檳榔塊刺激後第二型基質金屬蛋白酶在口腔細胞的表現情形

劉巡宇(Shyun-Yeu Liu) ； 林美惠(Mei-Huei Lin) ； 楊舜鈞(Shun-Chun Yang) ； 黃冠誠(Guan-Cheng Huang) ； 張王麗雪(Lisa Chang) ； 張善鈞(Sam Chang) ； 顔欽堉(Ching-Yu Yen) ； 蔣維凡(Wei-Fan Chiang) ； 郭永煙(Yung-Yen Kuo) ； 陳瑮琳(Li-Lin Chen) ； 李今海(Chin-Hai Lee)

《Journal of the Formosan Medical Association》 104卷6期 (2005/06) Pp. 390-397

https://doi.org/10.29828/JFMA.200506.0001

並列摘要

Background and Purpose: Arecoline, an areca quid, (AQ) component, has been shown to inhibit the secretion and activity ofmatrixitetalloproteinase-2(MMP-2) in fibroblast cultures. This study assessed whether MMP-2 expression was inhibited in the saliva samples and tumor specimens of oral tumor patients with a long-term history of AQ consumption. The net effect of crude AQ extract (AQE) on MMP-2 expression by oral cells was also investigated. Methods: Western blot analysis, zymography, and reverse transcriptase-polymerase chain reaction were used to detect MMP2 protein and mRNA in saliva and tumor samples, as well as in the conditioned media (CM) of oral cell cultures. Results: The level of MMP-2 protein was significantly higher in the saliva samples of 12 oral tumor patients who had a minimum 10-year AQ-consuming history than in those of 12 non-AQ-using healthy controls (p＜0.05). MMP-2 mRNA was expressed in 26 of 28 oral squamous cell carcinoma (OSCC) specimens. MMP-2 protein was also detectable in the tested OSCC homogenates. Short-term stimulation with 10% AQE increased he secretion of MMP-2 protein in the CM of oral epidermoid carcinoma cell Meng-1 (an OSCC cell line) and oral fibroblasts.
Conclusions: MMP-2 expression is elevated rather than inhibited in most oral tumor patients with long-term AQ usage. Short-term AQE stimulation also increases the secretion of MMP-2 by oral epithelial cells and fibroblasts. Our results suggest that AQ consumption may promote oral tumor progression through the induction of MMP-2 secretion.

並列關鍵字

Areca ； Carcinoma, squamous cell ； Fibroblasts ； Matrix metalloproteinases