In Vitro Osteoblastic Differentiation of Rabbit Mesenchymal Stem Cells in a Poly (Lactide-Co-Glycolide)/Interpore/Collagen Composite System

Purpose: Bone grafting is widely used in clinical orthopedic surgery. In order to avoid the complications associated with autogenous bone grafting, many kinds of bone substitutes have been developed. We developed an MSC carrier comprising a hot compression-molded polylactide/polyglycolide (PLGA), a porous calcium phosphate product that is used clinically and commercially (Interpore; Pro-Osteon 500R, Irvine, California) and a type I collagen composite for use in bone grafting. The purpose of this study was to evaluate the osteoblastic differentiation ability of rabbit MSC s loaded on this PLGA /Interpore/type I collagen carrier system in vitro. Materials & Methods: MSC s aspirated from rabbit bone marrow were cultured in induction medium and loaded onto the PLGA /Interpore/type I collagen carrier system. After culture for 7 and 14 days, the osteoblastic differentiation ability was tested using histological analysis and reverse transcription polymerase chain reaction (RT-PCR ) and by measuring the calcium and alkaline phosphatase levels. Results: After culture in the induction medium for 7 and 14 days, both the calcium and alkaline phosphatase levels were significantly higher than those in the control group. RT -PCR revealed mRNA expression of osteopontin, core binding factor alpha 1 (Cbfa1), and type I collagen. Optical micrographs of the MSC-loaded PLGA /Interpore/type I collagen carrier surface were obtained. After culture in osteogenic medium for 14 days, the carrier surface was almost entirely covered by cells and a dense collagenous extracellular matrix. Conclusions: The results of the study suggest that the biodegradable PLGA / Interpore/type I collagen MSC carrier system possesses potential osteoblastic differentiation ability.

並列關鍵字

mesenchymal stem cell ； bone substitute ； PLGA ； collagen ； interpore

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