高脂飼料對於倉鼠腸道總菌相組成的影響

Effect of High-fat Diet on Intestinal Microbiota of Hamster

蔡佩菁(Pei-Ching Tsai) ； 施亞凡(Ya-Fun Shih) ； 陳星光(Hsin-Kuang Chen) ； 張智閔(Chih-Min Chang) ； 郭家芬(Chia-Feng Kuo)

《台灣營養學會雜誌》 43卷4期 (2019/12) Pp. 100-110

https://doi.org/10.6691/NSJ.201912_43(4).0001

摘要

本研究主要是利用次世代定序系統與大數據分析方法，研究高脂飲食對倉鼠全腸道總菌相組成變化的影響。將倉鼠分為控制組與高脂組各20隻，並於第4、8、12、20週分別犧牲各組3隻倉鼠，分析血液生化值和糞便菌相的變化情形，其中第20週高脂組為餵飼高脂飼料至第12週後改餵飼對照組飼料8週。結果顯示攝取4－12週的高脂飼料後，糞便中有6個菌種所佔的比例較控制組減少約2.3～90.5倍，其中包括芽孢梭菌（Clostridium hiranonis）、普拉梭菌（Faecalibacterium prausnitzii）、普氏菌（Prevotella copri）、類桿菌（Bacteroides plebeius）、柯林氏菌（Collinsella stercoris）和痤瘡丙酸桿菌（Propionibacterium acnes）；而有2個菌種所佔的比例增加2.3～8.0倍，其中包括容易引起發炎反應的脫硫弧菌（Desulfovibrio C21 C20）和高山姬鼠螺旋桿菌（Helicobacter apodemus）。高脂組在第12週後停止攝取高脂飼料並改為對照組的飼料餵食8週後，結果顯示除了柯林氏菌含量仍較低外，其他原本減少或增加的菌種與控制組比較起來則無顯著差異。實驗結果顯示飲食中的脂肪含量的確影響腸道菌相的組成。

關鍵字

腸道微生物 ； 高脂飲食 ； 次世代定序 ； 倉鼠

並列摘要

Next generation sequencing and big data analysis were applied to study the effect of high-fat diet on intestinal microbiota of hamsters. Forty male 8-week old Syrian hamsters were divided into control and high-fat groups. Three animals from each group were sacrificed at week 4, 8, 12, 20 weeks to analyze blood parameters and fecal microbiota. Microbiota analysis indicates high-fat diet promoted 6 strains by 2.3-90.5 folds, including Clostridium hiranonis, Faecalibacterium prausnitzi, Prevotella copri, Bacteroides plebeius, Collinsella stercoris, and Propionibacterium acnes. Two strains were increased by high-fat diet by 2.3-8.0 folds, including Desulfovibrio C21 C20 and Helicobacter apodemus which cause inflammation. After stopping the intake of high-fat diet for 8 weeks, the strains in animals of ＂high-fat＂ group were not significantly different from those of control group, except Collinsella stercoris was still lower in high-fat group. The results indicate that intestinal microbiota is affected by dietary fats.

並列關鍵字

intestinal microbiota ； high-fat diet ； next generation sequencing ； hamster