In the age of advanced medical advancement and technology, people attach more importance on real-time health management systems. Nowadays, sportswear emphasize on fashion, function and biomedical signals. This study use stitch of Plated, Inlay, the repeat knit stitches, and interval of tuck stitches techniques to design fabrics and make various fabric structure stitching arrangement, as well as research the conductivity transmission performance of conductive yarns in knitted fabrics. 45 knitted fabrics are design and tested for electrical resistance. The results of the study, considering the cost of conductive yarns, choose fabrics with 1 interval of tuck stitches, plated fabrics with 2 intervals of conductive yarn stitches, and 1 intervals of polyester stitches. The resistance of the sample is 0.44Ω. 707.0cm of silver yarns were used in the sample, which not only reduced the amount of silver yarns used, but also achieved the benefits of better conductivity and biomedical signal transmission performance.