超音波法快速萃取及定量葉綠素

Rapid Extraction and Determination of Chlorophyll Using Ultrasonics

張上鎮(Shang-Tzen Chang) ； 王升陽(Sheng-Yang Wang) ； 葉汀峰(Ting-Feng Yeh) ； 吳季玲(Chi-Lin Wu)

《台灣林業科學》 12卷3期 (1997/09) Pp. 329-334

https://doi.org/10.7075/TJFS.199709.0329

摘要

為了改良葉綠素萃取方法，本研究嘗試利用超音波法配合丙李玉酮、DMF(Dimethylformamide)及DMSO(Dimethyl sulfoxide)三種有機溶劑萃取樟樹葉片之葉綠素，然後以紫外－可見光光譜儀分析，評估萃取方法與萃取溶劑對萃取效果之影響，並探討光譜曲線之變化，同時計算葉綠素之含量。試驗結果顯示，最佳的萃取方法係將葉片之粉末（小於0.7mm）置於DMF溶劑中，並以超音波法取代傳統研磨法，萃取3分鐘後即可。此法操作簡易且萃取快速，除可獲得較佳的萃取效果外，並可增加試驗分析的準確性。與葉綠素在丙酮溶劑中的光譜特性吸收峰位置相比較，葉綠素的光譜特性吸收峰在DMF與DMSO兩種溶劑中顯現了向紅位移。以DMSO、80%丙酮與DMF三種溶劑萃取出來之葉綠素含量分別為l.765mg/g、2.061mg/g與3.628mg/g。

關鍵字

樟樹 ； 葉綠素 ； 萃取 ； 研磨法 ； 超音波法 ； 離心機法 ； 向紅位移

並列摘要

The purpose of this study is to establish a simple method for chlorophyll extraction using ultrasonics. The leaf chlorophyll of Cinnamomum camphora was extracted with acetone, DMF, or DMSO using ultrasonics. The chlorophyll extracts were analyzed with a UV-Visible spectrophotometer. The spectroscopic characteristics of 3 chlorophyll extracts were compared and chlorophyll content was quantified. The experimental results demonstrate that extraction of leaf powders (particle diameters smaller than 0.7mm) with DMF by ultrasonics for 3 min is a rapid, convenient, and effective method for extracting chlorophyll. Compared with the absorption positions of chlorophyll in acetone, a bathochromic shift was observed at the absorption peaks of chlorophyll in DMF and DMSO. The chlorophyll contents extracted with DMSO, 80% acetone, and DMF were 1.765mg/g, 2.061mg/g, and 3.628mg/g, respectively.

並列關鍵字

Cinnamomum camphora ； chlorophyll ； extraction ； grinding method ； ultrasonic method ； centrifugation method ； bathochromic shift