Purpose: Intensive care unit (ICU) training for nurses and physicians are required to develop a variety holistic approaching skill in managing emergent and challenging patients and families. Facing the heightened needs of ICU, nurses and physicians need to assess patients and integrate the opinion of healthcare team by communication and collaboration. So, ICU nurses and physicians are expected to be highly experienced and knowledgeable persons that familiar with emergent and holistic care. Simulation had been suggested as an assessment tool for evaluating the effects of educational intervention. Methods: In this study, according to the experience and adult learning theory, we used post-neonatal resuscitation program (NRP) in situ simulation for evaluating the effectiveness of NRP training. Meanwhile, the core elements of holistic approach skills including history taking, physical examination, communication and teamwork were incorporated in the program. Results: In this study, post-NRP simulation was used as an assessment tool to evaluate the effectiveness of NRP through assessments by instructors and participants. Notably, this pilot intervention program demonstrated the feasibility of a holistic care simulation program in newborn ICU. Conclusions: In situ simulation and assessment affords experimental learning without compromising patient safety. It is a novel training that could be integrated into regular ICU intervention.