Antifungal Activity of Freshly Squeezed Garlic as Denture Cleanser on Candida Albicans Growth

Intan Nirwana ； Titien Hary Agustantina ； Alhidayati Asyma

《Journal of International Dental and Medical Research》 11卷2期 (2018/07) Pp. 639-642

摘要

The objective of this study was to investigate the growth of Candida albicans on acrylic resin plate after soaking in freshly squeezed garlic. Samples were immersed in sterile saliva for 1 hour at room temperature for the formation of pellicle. They were taken and rinsed with phosphate buffer saline (PBS) solution, contaminated with Candida albicans by insertion into a tube containing Candida albicans suspension, incubated for 24 hours at 370 C, removed and rinsed with PBS solution, soaked in freshly squeezed garlic in test tubes for 3 minutes, containing 10 ml of freshly squeezed garlic of 20%, 30 %, 40 % concentration each and sterile distilled water. Samples were taken and rinsed with PBS, inserted into Sabouraud's broth, vibrated with vortex for 30 seconds to release Candida albicans attached to them. Suspension from Sabouraud's broth was taken, dripped on Sabouraud's dextrose agar, spreading, incubated for 48 hours, at 370C. Candida albicans colonies were calculated. Data were analysed using Anova and followed by LSD test. The Results showed that there were significant difference among the groups (p ＜ 0.05). In conclusion, the higher concentration of freshly squeezed garlic the lower the number of Candida albicans colonies.

關鍵字

Antifungal ； freshly squeezed garlic ； Candida albicans ； acrylic resin