Citric Acid-Polyurethane-Chitosan-Based Innovative Biocomposites as Candidates of Antibacterial Dialyzer Membrane

Evlyn Anggraini Santoso ； Prihartini Widiyanti ； Fulky A'yunni ； Fathania Nabilla ； Novita Putri Rahayu ； Adita Wardani Rahmania

《Journal of International Dental and Medical Research》 11卷2期 (2018/07) Pp. 718-722

摘要

Hemodialysis treatment is a replacement therapy renal function. Hemodialysis therapy with reuse system causes a risk of bacterial infection. In addition, routine vascular access by hemodialysis patients also factors of bacterial contamination which can increase the comorbidity and mortality rates of patients. This research aims to obtain biocompatibility and antibacterial properties by adding concentration chitosan in three variations. The functional group test showed the presence of a bond between PU-CA-PES-CS at a certain wavelength. SEM test proved that the pores produced on dialyzer membrane are still within the size range for the hemodialysis application at 0.001-0.1 μm. Hemolysis test results showed that the percentage of hemolysis in Sample A, Sample B, Sample C, and Sample D were 4.18%, 3.34%, 2.30% and 1.67% respectively. The percentage of hemolysis at less 5% is safe for contact with blood. Antibacterial test results revealed that PES-PU-CA membrane samples coated with chitosan variation are antibacterial. It can be seen from the formation of clear zone around the sample disc with diameter of 6,946-11,086 mm. PES-PU-CA dialyzer membrane coated with chitosan is a safe composite and potentially applicable as an antibacterial dialyzer membrane based on functional group test, antibacterial test, hemolysis assay, SEM, and cytotoxicity test.

關鍵字

Membrane dialyzer ； antibacterial ； polyurethane ； polyethersulfone ； chitosan.