開發電化學方法應用於食品中苯甲酸之定量檢測

苯甲酸為食品中常用之防腐劑，人體食入後可自行解毒排出，故符合標準者對人體無害，若是過量食入則易引起心跳快、流口水、腹瀉、皮膚過敏等症狀；台灣衛生福利部食品藥物管理署限制苯甲酸用量為0.6-2.0 g/kg以下。傳統檢驗方法具有操作過程繁瑣且耗時等缺點，因此本研究是應用電化學系統建立一個簡便並快速之方法定量檢測苯甲酸，實驗以未修飾之白金電極針對苯甲酸進行直接檢測，以循環伏安法探討檢測之最適條件，其最適掃描速率為100 mV/s，反應時間為30秒，反應槽溶液為0.04 M之Britton-Robinson緩衝溶液，pH值為2.0，最適溫度為45℃，以此條件製作檢測苯甲酸之檢量線，其線性範圍為2.5 × 10^(-5) M至1.6 × 10^(-3) M，線性迴歸方程式為y = 0.02575 x + 0.32717，R^2值為0.9977。以此電化學系統檢測實際樣品，於碳酸飲料之回收率為97.74%到98.47%，代表以電化學方法定量碳酸飲料中之苯甲酸含量，具有直接檢測之可行性，同時亦具有操作簡單、反應快速、成本較為低廉等優點。

關鍵字

電化學感測器；防腐劑；苯甲酸；循環伏安法

並列摘要

Benzoic acid is a legal preservative that has been widely used in foods to prevent the growth of microorganisms. Nevertheless, excessive addition of benzoic acid may be harmful to consumers. Taiwan Food and Drug Administration (TFDA) has regulated requirement standard for use of food additives, not more than 0.6-2.0 g/kg calculated as benzoic acid. Traditional methods for detection of benzoic acid in food samples are not only complicated, but also time consuming. Therefore, the electrochemical sensor with non-modified electrode was developed and used for the measurement of benzoic acid in foods. The optimal operating conditions for the detector based upon cyclic voltammetry were determined as follows: platinum electrode was used as the working electrode, 0.04 M Britton-Robinson buffer (pH 2.0) was found to be suitable for this system, and the temperature was set as 45℃, with a scan rate of 100 mV/s, response time was 30 s. The sensor responded linearly to benzoic acid in a concentration range from 2.5 × 10^(-5) M to 1.6 × 10^(-3) M with correlation coefficient of 0.9977. After a recovery test, in carbonate beverage, the value of recovery was between 97.74% and 98.47%, suggesting that the sensor was feasible to detect benzoic acid directly in food samples. Compared with traditional methods, the detecting procedures of the sensor are simpler and the response time is shorter. Moreover, the cost of detection for benzoic acid can be reduced.

並列關鍵字

Electrochemical sensor ； Preservatives ； Benzoic acid ； Cyclic voltammetry

國際替代計量

開發電化學方法應用於食品中苯甲酸之定量檢測

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