Induction of MMP-9 in Mouse Cerebellum with Purkinje Cell Loss Caused by Angiostrongylus Cantonensis

小白鼠感染廣東住血線蟲誘發基質金屬蛋白酶9與小腦浦金氏細胞喪失之關係

賴世展(Shih-Chan Lai) ； 陳科銘(Ke-Min Chen) ； 楊仁邦(Jen-Pang Yang) ； 劉嘉鵬(Jia-Ping Liu) ； 徐程鈞(Cheng-Chun Hsu) ； 李宇心(Yu-Hsin Lee) ； 任思穎(Szu-Ying Jen) ； 黃榮緯(Jung-Wei Huang) ； 李秀雄(Hsiu-Hsiung Lee)

《中山醫學雜誌》 14卷4期 (2003/12) Pp. 485-493

https://doi.org/10.30096/CSMJ.200312.0001

摘要

廣東住血線蟲症（angiostrongyliasis）引起之神經系統疾病是由於發育中的廣東住血線蟲（Angiostrongylus cantonensis）侵入中樞神經系統所造成。在感染廣東住血線蟲的BALB/c strain小白鼠發現小腦浦金氏（Purkinje）細胞出現退化性萎縮及部分喪失的現象。基質金屬蛋白酶9（matrix metalloproteinase-9，MMP-9）mRNA的表現及酵素活性只在感染廣東住血線蟲的小白鼠小腦檢測到，經西方點墨法（Western blotting）確定此酵素為MMP-9，而在未感染的小白鼠則檢測不到此酵素。另外，組織免疫試驗亦證實MMP-9定位在退化的浦金氏細胞。這些結果顯示MMP-9可能與小白鼠感染廣東住血線蟲引起小腦浦金氏細胞喪失有關。

關鍵字

廣東住血線蟲 ； 基質金屬蛋白酶9 ； 浦金氏細胞

並列摘要

Neurological disorders in angiostrongyliasis are caused by invasion of the central nervous system by developing Angiostrongylus cantonensis larvae. After A. cantonensis infection, the cerebellums of BALB/c strain mice were observed to have a loss of Purkinje cells. Histologically, the Purkinje cells in the infected mice cerebellum were shown to have degenerative atrophy or partial loss. The matrix metalloproteinase-9 (MMP-9) mRNA and activity was induced in the infected mice, and the enzyme was absent in uninfected mice. Western blotting showed that the anti-MMP-9 antibody recognized a single 94 kDa protein from A. cantonensis-infected mice. Immunohistochemistry also showed localization of MMP-9 within the degenerative Purkinje cells. These results suggest MMP-9 in mice infected with A. cantonensis may be related to the loss of Purkinje cells.

並列關鍵字

Angiostrongylus cantonensis ； matrix metalloproteinase 9 ； Purkinje cell