市售蔓荊子藥材之鑑別及其化學成分含量之測定

Identification the Authenficity of Viticis Simplicifoliae Fructus by Pharmacognosy and Determination the Content of Casticin by HPLC

陳佩儀(Pei-Yi Chen) ； 劉宜祝(Yi-Chu Liu) ； 羅吉方(Chi-Fang Lo)

《食品藥物研究年報》 2期 (2011/12) Pp. 367-373

https://doi.org/10.6945/ARFDR.201112.0369

摘要

蔓荊子（Viticis Simplicifoliae Fructus）為馬鞭草科植物單葉蔓荊Vitex trifolia L. var.simplicifolia Cham.或蔓荊Vitex trifolia L.之乾燥成熟果實。中華中藥典尚未制定規範蔓荊子藥材之指標成分含量基準，為了解市售蔓荊子之基原及其指標成分含量，本計畫價購全國北、中、南區藥廠及藥房蔓荊子藥材共52件，參照文獻之記載，經性狀特徵、組織鏡檢及薄層層析法鑑別，52件檢體皆為正品蔓荊子藥材，再以高效能液相層析法（HPLC）分析檢測藥材中蔓荊子黃素（Casticin）之含量。本實驗之高效能液相層析法係採用cosmosil 5C18 AR II，4.6×250mm，5μm管柱，流動相為乙腈：0.1%磷酸水溶液（60：40）等梯度沖提，檢測波長為258nm。線性迴歸方程式Y= 62.657X－0.0012 （r^2 = 0.9999），呈良好線性關係。市售蔓荊子藥材52件，利用上述高效能液相層析法分析蔓荊子黃素含量介於0.003－0.071%之間，平均值為0.015%。

關鍵字

蔓荊子 ； 蔓荊子黃素 ； HPLC ； 指標成分含量

並列摘要

The botanical origin of Viticis Simplicifoliae Fructus is the mature and dried fruit of Vitex trifolia L. var. simplicifolia Cham. and Vitex trifolia L. The quantitation and regulation of the index ingredient have not been established in the China Chinese Medicine Pharmacopoeia. In this study, 52 samples were purchased from Taiwan market, which were compared to authentic materials by morphology, microscopy and thin layer chromatography. The results showed that all of 52 samples (100%) were Viticis Simplicifoliae Fructus.The amount of the index ingredient, casticin, in crude drugs was determined by high performance liquid chromatography. Samples were analyzed over a Cosmosil 5C18 AR II reversed phase column (5 μm) using isocratic elution with acetonitrile and 0.1% phosphoric acid solution (60 : 40) and monitored at 258 nm absorbance. Regression equations revealed a linear relationship (r2 = 0.9999) between the peak-area ratios of casticin to internal standard and the amount of casticin in crude drugs. The contents of casticin in 52 samples of Viticis Simplicifoliae Fructus were 0.003-0.071% with a mean content of 0.015%.

並列關鍵字

Viticis Simplicifoliae Fructus ； casticin ； HPLC ； pharmacognoy