應用rbcL基因序列鑑別桑寄生及其誤用藥材

rbcL（ribulose-1，5-bisphosphate carboxylase/oxygenase large subunit）基因是植物葉綠體中與光合作用相關的重要基因之ㄧ，為植物分類上常用的DNA標記（DNA marker），也應用於DNA barcode上。本研究利用rbcL基因序列鑑別中藥中經常發生誤用的桑寄生藥材。桑寄生為桑寄生科（Loranthaceae）植物桑寄生Taxillus chinensis的乾燥帶葉莖枝，常見誤用品有紅花寄生（T. parasiticus）、槲寄生（Viscum coloratum）、楓寄生（V. liquidambaricolum）及桑科的桑枝（Morus indica），研究中以確認基原的對照藥材序列及參考GenBank資料庫，建立標準序列，再收集市售及向藥廠價購之桑寄生藥材檢體27件，抽取DNA後，以rbcL基因的universal primers進行PCR及DNA定序分析，序列結果比對標準序列，得到鑑定結果，結果顯示27件檢體鑑定為桑寄生正品12件、紅花寄生11件、槲寄生2件及桑枝2件。

關鍵字

桑寄生； rbcL ； DNA定序

並列摘要

rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit) is the important component of chloroplast for photosynthesis. The gene of rbcL is a useful DNA marker for taxonomy and DNA barcode. In this study, we discriminated Taxilli Ramulus from its counterfeits by the sequences of rbcL gene. Taxilli Ramulus, the dried stems with leaves of Taxillus chinensis, is widely used in Chinese medicine. Taxilli Ramulus is easy confused with the dried stems of T. parasiticus, Viscum coloratum, V. liquidambaricolum and Morus indica by appearance. We established a database of rbcL sequence of Taxillus chinensis and its counterfeits with the sequences of authentic raw materials and the data from GenBank. Our samples were purchased from local traditional Chinese (herbal) pharmacies and markets. The extracted DNA of samples was amplified by PCR with universal primers for rbcL gene, and then analyzed on an auto-sequencer. The revealed sequences were compared with the database. Twelve out of 27 samples were identified as T. chinensis, 11 T. parasiticus, 2 V. coloratum and 2 M. indica.

並列關鍵字

rbcL ； Taxilli Ramulus ； DNA sequencing

國際替代計量

應用rbcL基因序列鑑別桑寄生及其誤用藥材

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