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液相層析法分析茶飲料中兒茶素

Determination of Catechins in Tea Drinks by High Performance Liquid Chromatography

摘要


本研究係建立利用高效液相層析儀(high performance liquid chromatograph,HPLC)配合光二極體陣列偵測器(photodiode array detector,PDA),檢測茶飲料中8種兒茶素之檢驗方法。檢體經稀釋、濾膜過濾後即可以Agilent Eclipse Plus C18(5μm,內徑4.6mm×25cm)液相層析管柱,配合添加0.1%甲酸之去離子水及乙腈溶液作為移動相梯度之條件下,依流速1mL/min以高效液相層析儀配合光二極體陣列偵測器於波長280nm進行檢測,可將8種兒茶素與咖啡因(caffeine)分離。進行22件茶飲料健康食品之兒茶素標示符合性之評估,其兒茶素檢測含量範圍介於24-96mg/mL之間,除年初抽驗之4件茶飲料檢體外,其餘陸續抽驗之18件檢體,其兒茶素檢出量均與標示相符,顯示茶飲料兒茶素標示不符之情形確有改善。

並列摘要


A method was developed for the determination catechins in tea drinks by high performance liquid chromatography (HPLC) with photodiode array detector (PAD). Samples were diluted with 0.1% phosphoric acid solution appropriately, eluted in stepwise with 0.1% formic acid (prepared with deionized water) and acetonitrile at flow rate 1 mL/min through an Agilent Eclipse Plus C18 column (4.6 mm i.d. × 25 cm, 5 μm) and detected at 280 nm wavelength. Eight kinds of catechins and caffeine in tea drinks were well separated. The amounts of catechins detected from 22 samples were in the range of 24-96 mg/mL. Except for 4 samples, the amounts of catechins detected in the rest 18 samples were all coincided with the labeling regulation.

並列關鍵字

tea drink catechin caffeine HPLC

被引用紀錄


周士會(2007)。添加菊苣糖之綠茶對大白鼠腸道菌相之影響〔碩士論文,臺北醫學大學〕。華藝線上圖書館。https://doi.org/10.6831/TMU.2007.00127
羅利巧(2015)。茶湯水色與茶湯內容物含量之關係〔碩士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2015.11074

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