- 期刊
評估不同廠牌的Cetrimide Agar與Cetrimide Nalidixic Acid Agar (CN)以及CompactDry PA與M-PA-C Agar應用於濾膜法檢測水中綠膿桿菌的效能
Evaluation the Efficacy of Different Brands of Cetrimide Agar, Cetrimide Nalidixic Acid Agar (CN), CompactDry PA, and M-PA-C Agar Used in the Filter Membrane Method for Testing Pseudomonas aeruginosa in Bottled and Packaged Drinking Water
摘要
對於營養要求不高的綠膿桿菌（銅綠假單胞菌；Pseudomonas aeruginosa），常存於在潮濕環境當中，常為醫院感染菌源之一，也常污染包裝飲用水及瓶裝水，因此，台灣衛福部食品藥物管理署及其他國家衛生主管單位將其列為瓶裝水的污染指標菌，各國大多利用濾膜法將水檢體過濾，但濾膜所貼的培養基各有不同，包括cetrimide agar、cetrimide nalidixic acid agar (CN)、M-PA-C agar或其它特殊設計的裝置。另外，在各國藥典（USP、EP、中華藥典、中華人民共和國藥典）檢測產品的綠膿桿菌時，則均建議使用培養基cetrimide agar。為了瞭解不同廠牌CN agar與cetrimide agar及其它特殊設計培養基對綠膿桿菌的檢測效能，本研究利用濾膜法過濾含有相同濃度（菌量）的純化綠膿桿菌，然後分別將濾膜貼在5種cetrimide agar、5種CN agar及Mueller-Hinton II agar (M-H agar)、CompactDry PA與M-PA-C agar，經過培養18-24小時後，以M-H agar的生長菌落數為基準進行回收率評估。結果發現除了A及B廠牌的cetrimide agar及F廠牌CN agar平均回收率均低於80%，其餘測試的各種培養基均有良好的回收率(80-120%)。在菌落顯色方面，僅有D廠牌的cetrimide agar及I廠牌的CN agar上生長的綠膿桿菌菌落呈現非典型的粉膚色，而其它廠牌者則呈藍綠色，因此，在濾膜法中經生長效能確效的各個廠牌CN agar、cetrimide agar以及CompactDry PA與M-PA-C agar均能有效率的分離、鑑別及計數綠膿桿菌。
並列摘要
Pseudomonas aeruginosa, an organism with low requirements for nutrition, is one of the bacteria commonly found in humid environments. Relatedly, it is frequently found to be involved in the contamination of bottled and packaged drinking water. Therefore, this organism has been identified as an indicator organism for bottled water by the Bureau of Food and Drug Administration (FDA), Ministry of Health and Welfare, Executive Yuan, of Taiwan, as well as by the health authorities of many other countries. In this study, the filter membrane method was employed for the filtering of water samples, after which the samples were applied on a plate medium consisting of M-PA-C (Creative Co., Taiwan), cetrimide, cetrimide nalidixic acid agar (CN), CompactDry PA (Nishui), or another specialized medium. In addition, P. aeruginosa tested methods promulgated by pharmacopeia of different countries or organizations (USP, EP, Chinese pharmacopeia of the ROC and the pharmacopeia of the PROC) in which the media recomm ended was cetrimide agar. To explore the efficacy of different brands of cetrimide agar, CN, and other specialized media, this study used the filter membrane method to filter water with same concentrations (quantities) of P. aeruginosa, and then applied the membrane with 5 different brands of cetrimide agar and 5 brands of CN agar as well as Mueller-Hinton (M-H) agar, CompactDry PA , and M-PA-C agar. After incubation, the recovery rates of P. aeruginosa, compared with that of the control M-H agar, were calculated for all the tested media. The results indicated that, other than the recovery rates of the A and B brands of cetrimide agar and the F brand of CN being lower than 80%, all the other tested media had excellent recovery rates (that is, rates of 80% to 120%). Regarding the pigment produced by P. aeruginosa, only CN agar and cetrimide agar from one manufacturer located in the Mainland China appeared pink in color, whereas all the other tested brands were blue-green in color. We therefore concluded that the validated CN agar and cetrimide agar brands, as well as CompactDry PA and M-PA-C agar, can differentiate, isolate and count P. aeruginosa effectively.