綠色製程製備源自天然植物之螢光碳量子點與生物醫學應用

Luminescence Carbon Nanodots Derived from Natural Plants through Eco-friendly Synthesis for Biomedical

陳昱佑(Yu-Yu Chen) ； 樊孝汧(Siao-Cian Fan) ； 陳煥綸(Huan-Luen Chen) ； 莊宗原(Tzong-Yuan Juang)

《化學》 79卷3期 (2021/09) Pp. 201-208

https://doi.org/10.6623/chem.202109_79(3).001

摘要

本文以天然植物透過燒結與水熱法製備螢光碳量子點，開發多種碳源的前驅物如碳化竹子、碳化木頭、農產品廢棄物與中草藥衍生之碳量子點，具有製備方式簡單與環保優點。透過螢光光譜儀分析碳量子點具有螢光性質，穿透式電子顯微鏡觀察碳量子點尺寸為3-5 nm。另外，穿透式電子顯微鏡與X-ray繞射儀更說明碳量子點之結構為介於有序到無序碳結構之間，於傅立葉轉換紅外光譜儀與化學分析電子能譜儀鑑定碳量子點具有許多不同的含氧或含氮的官能基。細胞實驗中碳量子點表現出在濃度500μg/mL時，對人類皮膚角質細胞（HaCa Tcell）與人類乳腺癌細胞（MCF-7 cell）具低毒性，其細胞存活率皆超過80%。此外，碳量子點與細胞培養24小時後，利用共軛焦顯微鏡可以觀察到碳量子點會進入人類皮膚角質細胞與人類乳腺癌細胞內。

關鍵字

碳量子點 ； 綠色製程 ； 水熱法 ； 燒結法 ； 低生物毒性 ； 細胞顯影

並列摘要

Our carbon nanodots (CNDs) were prepared from natural plants through carbonization of bamboo and wood, and through hydrothermal synthesis of herbs and agricultural waste in facile and eco-friendly process. The resulting CNDs possessed spherical appearance between 3-5 nm nanoscale, and fluorescence behavior. TEM and XRD revealed the carbon nanostructure possess order and disorder lattice due to broad 2θ peak between 20° to 30°. Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy indicated our CNDs were divided into high oxygen-containing CNDs and nitrogen-doped CNDs due to different sources and processes. Cytotoxicity assays for CNDs-treated human keratinocyte HaCaT cells and human MCF-7 breast cancer cells revealed these CNDs behavior cell viability of 80% at 500 μg/mL. Moreover, confocal cell images showed fluorescent CNDs were located inside MCF-7 cells and HaCaT cells after treating CND for 24 h.

並列關鍵字

Carbon nanodot ； Eco-friendly ； Hydrothermal process ； Carbonized process ； Low cytotoxicity ； Bioimage