The purpose of this research is to investigate the effect of cooling and hyperoxia application on the circulatory and metabolism system during rest period of high intensity interval training (HIIT). This research included six healthy male participants (age 23.00 ± 2.02 years) who completed two high intensity interval rowing training session with cooling or hyperoxia application in counterbalance order. The HIIT contained 2 × 2 × 300 m of rowing. The rest period between sets was 10 min and the rest between every 300 m rowing in each sets was 90 s. The intensity was set to 95% of individual maximal power output (376.33 ± 136.72 watt). During the 10 min rest, either 2 × 90 s cooling (-120 - -140°C) or 8 min hyperoxia air (80% O_2) were applied. Capillary blood lactate (La), heart rate (HR), blood ammonia (NH_3), rating of perceived exertion (RPE), and venous blood gas (pO_2, pCO_2, pH, HCO_3, and sO_2) were measured. The results showed that the concentration of blood lactate after HIIT was higher in hyperoxia than in cooling application (p > .05). During the 10 min rest between training sets, ammonia increased more in cooling application than in hyperoxia (p < .05). pO_2 and sO_2 at R8 were higher in hyperoxia than in cooling application. In addition, HCO_3 at R8 is lower in hyperoxia. In conclusion, hyperoxia application helps to regulate heart pressure, increase partial pressure of oxygen and oxygen saturation, and delay lactic acid and blood ammonia concentration in the subsequent exercise, while cooling contributes to excluding lactic acid in recovery period.