粉枝藻在各種培養條件下所產生的佛羅里多苷的含量變化

Responses of floridoside under various culturing parameters in Liagora sp.

郭敬安(Ching-An Kuo)

指導教授 ： 李宗徽

國立臺灣大學/生命科學院/漁業科學研究所/碩士(2021年)

https://doi.org/10.6342/NTU202102945

摘要

佛羅里多苷 (floridoside) 是紅藻中的代謝產物之一，在結構上，是紅藻中主要的小分子糖苷，是短期的儲備碳庫並用於有機碳的轉運，將從光合作用得到的無機碳轉化為多醣和其他物質，並在調控紅藻中的滲透壓扮演著重要角色。該化合物在細胞中的合成和代謝受到各種生理狀況的調節，其含量也因環境條件和紅藻種類而異。本研究以大型紅藻粉枝藻 (Liagora sp.01) 的絲狀體 (filament) 作為研究材料，觀察粉枝藻在不同的溫度、鹽度和光質等生長條件下所產生佛羅里多苷的含量變化。在光質實驗中，在藍光照射下培養14天所產生的佛羅里多苷最多 (38.31 mg/g)，是對照組的1.3倍；在鹽度實驗中，高鹽組 (鹽度45‰) 培養7天所產生的佛羅里多苷最多 (69.82 mg/g)，是對照組 (36‰) 的1.95倍；在溫度實驗中，高溫 (32 °C) 培養3天所產生的佛羅里多苷達到51.6 mg/g，是對照組 (25 °C) 的3.25倍；高溫高鹽 (32 °C、鹽度45‰) 培養2天所產生的佛羅里多苷達到59.41 mg/g，是對照組 (25 °C) 的4.55倍。綜合以上結果，粉枝藻在高溫、高鹽和藍光的培養條件下會生產較多的佛羅里多苷以達到保護和滲透壓調節的作用。

關鍵字

粉枝藻 ； 佛羅里多苷 ； 溫度 ； 鹽度 ； 光質

並列摘要

Floridoside is one of the main metabolites in red algae. It is the main low-molecular-weight carbohydrates, and is involved in acclimation to osmotic changes in red algae. The synthesis and metabolism of the compound in cells are regulated by various physiological conditions, and its content also varies with environmental conditions and red algae species. In this study, the filaments of the Liagora sp.01 were used as the research material to observe the change in floridoside content under different environmental conditions such as temperature, salinity and light quality. In the light quality experiment, floridoside produced by culturing for 14 days under blue light irradiation were the most (38.31 mg/g), which was 1.3 times that of the control group. In the salinity experiment, the most floridoside produced by culturing for 7 days in the high-salt group (salinity 45‰) (69.82 mg/g), which is 1.95 times that of the control group (salinity 36‰). The most floridoside produced by culturing for 3 days at high temperature group (32 °C) (51.6 mg/g), which is 3.25 times that of the control group (25 °C). The most floridoside produced by culturing for 2 days at high temperature and high-salt group (salinity 36‰、32 °C) (59.41 mg/g), which is 4.55 times that of the control group (25 °C). Based on the above results, under the cultivation conditions of high temperature, high salt and blue light, Liagora sp. 01 will produce more floridoside to play a role in protection and osmotic regulation.

並列關鍵字

Liagora ； floridoside ； temperature ； salinity ； light quality

參考文獻

Arad, S. and Levy-Ontman, O. (2010) Red microalgal cell-wall polysaccharides: biotechnological aspects. Current Opinion in Biotechnology. 21(3): 358-364.

Ball, S., Colleoni, C., Cenci, U., Raj, J. N. and Tirtiaux, C. (2011). The evolution of glycogen and starch metabolism in eukaryotes gives molecular clues to understand the establishment of plastid endosymbiosis. Journal of Experimental Botany. 62 (6): 1775–1801.

Bondu, S., Cerantola, S., Kervarec, N. and Deslandes, E. (2009) Impact of the salt stress on the photosynthetic carbon flux and 13C-label distribution within floridoside and digeneaside in Solieria chordalis. Phytochemistry. 70(2): 173-184

Breitenbach, U., Kallmayer, V., Raschke, T., Scherner, C., Siefken, W. and Viala, S. (2006) Cosmeticpreparations containing glucosyl glycerides and one or moreacrylamidomethyl propylsulphonic acid polymers. WO 2006122669, Germany.

Chanda, B., Venugopal, S. C., Kulshrestha, S., Navarre, D. A., Downie, B., Vaillancourt, L., Kachroo, A. and Kachroo, P. (2008) Glycerol-3-phosphate levels are associated with basal resistance to the hemibiotrophic fungus Colletotrichum higginsianum in Arabidopsis. Plant Physiology. 147(4): 2017-2029