功能性近紅外光譜用於多層頭部組織模型的實驗驗證

Experimental Validation of Functional Near-infrared Spectroscopy Applied to Multi-layered Tissue Phantoms

蘇文偉(Wen-Wei Su)

指導教授 ： 宋孔彬

國立臺灣大學/電機資訊學院/生醫電子與資訊學研究所/碩士(2021年)

https://doi.org/10.6342/NTU202100992

摘要

本論文設計了多層組織仿體以模擬頭皮層與大腦灰質層的血液動力學變化，透過兩種吸收物質實驗來模擬血液中的帶氧血紅素與不帶氧血紅素。
建立寬頻近紅外光譜系統，搭配自製的光纖探頭與固定器，共有兩個SDS，根據系統分析結果，本研究之近紅外光系統具有相當的穩定性，且透過多層組織仿體光譜測量結果，驗證本系統具備測量多層組織模型的能力。
在多層組織仿體實驗，兩種吸收物質實驗，藉此模擬大腦經刺激的血液動力學的變化，透過MBLL去計算其濃度變化。根據實驗結果，本研究使用寬頻波長與挑選兩波長分析MBLL，分別分析改變L1實驗、只改變L4實驗、同時改變L1與L4實驗的濃度變化，以上三個分析結果，使用寬頻波長或兩個波長在三個分析各有好壞。
本論文建構的多層組織仿體提供了以實驗證據最佳化波長組合，相較於過去許多文獻僅使用模擬的方法來選取最佳波長組合，本論文的結果能夠直接且正確地反映實際的近紅外光譜系統在定量不均勻組織模型內的吸收物質濃度變化的性能。

關鍵字

漫反射光譜 ； 功能性近紅外光譜 ； 多層組織仿體 ； 血液動力學 ； Modified Beer Lambert Law(MBLL) ； 蒙地卡羅模擬

並列摘要

In this study, a multi-layer tissue phantom was designed to simulate the hemodynamic changes of the scalp layer and the gray matter layer of the brain. The two absorption substance experiments were used to simulate the oxygenated hemoglobin and the non-oxygenated hemoglobin in the blood.
In this study, a broadband near-infrared spectroscopy system was established, with a self-made fiber optic probe and fixator. There are two SDS in total. According to the results of system analysis, the near-infrared optical system in this study is quite stable, and the results of spectrum measurement through multi-layer tissue phantom , To verify that the system has the ability to measure multi-layer models.
In the multi-layer tissue phantom experiment, the experiment of two kinds of absorption substances simulates the changes in the hemodynamics of the brain after stimulation, and calculates its concentration changes through MBLL. According to the experimental results, this study uses broadband wavelengths and selects two wavelengths to analyze MBLL. The changes in the L1 experiment, only the L4 experiment, and the L1 and L4 experiments are changed at the same time. The above three analysis results use the broadband wavelength or two wavelengths. There are good and bad in each of the three analyses.
The multi-layer tissue phantom constructed in this study provides experimental evidence to optimize the wavelength combination. Compared with many previous literatures that only use simulation methods to select the best wavelength combination, the results of this paper can directly and accurately reflect the actual closeness. The performance of the infrared spectroscopy system in quantifying the changes in the concentration of absorbing substances in the heterogeneous tissue model.

並列關鍵字

Diffuse Reflectance Spectroscopy ； Functional Near-infrared Spectroscopy(fNIRS) ； Multi-layer Tissue Phntom ； Hemodynamic ； Modified Beer Lambert Law(MBLL) ； Monte Carlo simulation

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