Bifidobacterium longum 發酵乳改善胃腸道功能功效評估

Evaluation on the gastrointestinal function ameliorative effects of yogurt plus fermented with Bifidobacterium longum

胡凱鈞(Kai-Chun Hu)

指導教授 ： 潘子明

國立臺灣大學/生命科學院/生化科技學系/碩士(2016年)

https://doi.org/10.6342/NTU201610281

摘要

雙叉桿菌為常見之益生菌，目前已有許多文獻指出其具有改善腸道菌相、調節免疫及調節血脂等功效。本研究探討 Bifidobacterium longum 發酵乳對改善腸道細菌菌相及其衍生之功效。連續管餵 Sparque-Dawley (SD) 大鼠介入低、中、高劑量 (2.135、4.27、8.54 g/kg BW) 發酵乳凍乾粉共 8 週。結果顯示連續管餵 Sparque-Dawley (SD) 大鼠 Bifidobacterium longum 發酵乳凍乾粉第 2、4、6、8 週可顯著提升糞便及盲腸內容物中 Bifidobacterium spp.、Lactobacillus spp. 菌數，並顯著降低 Clostridium perfringens、Enterobacteriaceae 菌數，並增加放射性活性碳在小腸中的運輸率，抑制酸化酒精誘導之急性胃黏膜損傷的損傷指數。綜合以上結果，餵食不同劑量之 Bifidobacterium longum 發酵乳凍乾粉在腸道健康上可藉由改善腸道菌相，提升腸道運動，改善胃黏膜損傷情形，顯示Bifidobacterium longum 發酵乳為具有改善胃腸功能潛力之保健食品。

關鍵字

Bifidobacterium longum ； 腸道菌相 ； 腸道運動 ； 胃黏膜損傷

並列摘要

Bifidobacterium are frequently used as probiotics, and have been reported to improve intestinal microflora, modulate immune system, modulate blood lipid. This study investigated the effects of yogurt plus fermented with Bifidobacterium longum on intestinal microflora improvement. Sparque-Dawley (SD) rats were fed with 2.135、4.27、8.54 g/kg BW of freezed-dryed yogurt plus fermented with Bifidobacterium longum. Freezed-dryed yogurt plus fermented with Bifidobacterium longum administration 2nd、4th、6th、8th week significantly increased Bifidobacterium spp., Lactobacillus spp., and decreased Clostridium perfringens, Enterobacteriaceae in feces and cecum content. Moreover, increased intestinal transit of active carbon, and decreased lesion index in gastric mucosa of SD rats. These results demonstrated that administration of different dose of freezed-dryed yogurt plus fermented with Bifidobacterium longum has effects on improve intestinal microflora, gastrointestinal motility, prevent gastric mucosal lesion, suggesting that yogurt plus fermented with Bifidobacterium longum has ameliorative effects of gastrointestinal function.

並列關鍵字

Bifidobacterium longum ； intestinal microflora ； gastrointestinal motility ； gastric mucosal lesion

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