薏苡種皮乙醇萃取物對於子宮內膜癌之影響及其作用機制之探討

Effects of adlay testa ethanolic extract on the growth of endometrial cancer cells.

王韻雅(Yun-Ya Wang)

指導教授 ： 江文章

共同指導教授 ： 夏詩閔

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2012年)

https://doi.org/10.6342/NTU.2012.10569

摘要

子宮內膜癌在歐美是婦癌的首位，而台灣近年來其發病率呈現上升趨勢，若就女性生殖道癌症中發生率則是排名第三位，僅次於子宮頸癌及卵巢癌。過去研究指出薏苡籽實對於抗突變與抑制腫瘤具有正面的調節作用。本研究目的為將薏苡籽實萃取物分別給予子宮內膜癌細胞 HEC-1A與 RL95-2，評估其抑制活性並篩選有效次區分物指標化合物，並進一步探討其作用機制。結果顯示，薏苡種皮乙醇萃取物 (adlay testa ethanolic extract, ATE) 具顯著抑制效果，其中以乙酸乙酯區分層 (ethyl acetate fraction of ATE, ATE-EA) 活性最佳，其次區分物中以 ATE-EA-C、D、E、F 抑制活性較明顯。進一步利用各種化學組成分析方法定性ATE-EA後所得的結果做活性分析，發現成分中的酚類化合物、黃酮類化合物、固醇類化合物以及脂肪酸類化合物皆具有抑制癌細胞生長的效果；根據流式細胞儀分析以及西方轉漬法的實驗結果，ATE-EA可促使子宮內膜癌細胞走向凋亡，經由加入抑制劑的MTT assay結果顯示ATE-EA抑制RL95-2生長是走PI3K/PTEN/AKT signaling pathway以及MAPK / p38 signalling pathway；另外透過 wound-healing assay 得知ATE-EA具有降低 HEC-1A 轉移的功效。因此，本研究的結果顯示薏苡種皮乙醇萃取物具有抑制子宮內膜癌細胞生長的效果。

關鍵字

子宮內膜癌 ； 薏苡種皮 ； 凋亡

並列摘要

Endometrial cancer is the most common malignant tumor of gynecologic cancer in Western society. In recent years, the endometrial cancer’s incidence is increasing and became the third most common carcinoma of the female genital tract (behind ovarian and cervical cancer) in Taiwan. Adlay, an annual crop, has been reported with many health effect including anticancer. The purpose of this study is to investigate the inhibitory effects of extracts from adlay seeds on endometrial cancer cell HEC-1A (phosphatase and tensin homolog-positive) and RL95-2 (phosphatase and tensin homolog-negative) and also to investigate the mechanism. Further, the active fractions and effective components were elucidated. Results demonstrated that AT ethanolic extract (ATE) possessed significantly inhibitory capacity, and ethyl acetate fraction from ATE (ATE-EA) showed superior activity. C.D.E.F-sub-fraction from ATE-EA showed most favorable inhibitory potential in vitro. The amounts of effective components in ATE-EA were quantified by HPLC and GC/FID. The analytical results shown that the amount of phenolic compounds, flavonoids, steroids and fatty acid compounds in extracts have positive effect on anticancer avtivity. According to the flow cytometry and western blotting’s results, we found that ATE-EA suppressed HEC-1A and RL95-2 growth by inducing apoptosis. ATE-EA suppressed RL95-2 growth through PI3K/PTEN/AKT signaling pathway and MAPK / p38 signalling pathway. Furthermore, the wound-healing assay’s results demonstrated that ATE-EA inhibited migration in HEC-1A. Therefore, these present findings show the ability of ATE to inhibit endometrial cancer cell proliferation.

並列關鍵字

endometrial cancer ； adlay testa ； apoptosis

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