雞隻固醇調節區域結合蛋白之表現及其對脂質生合成基因之調控

The expression of sterol regulatory element binding protein and its function on regulating the expression of lipogenic genes in the chicken

柯雅慧(Ya-Huei Ko)

指導教授 ： 丁詩同

國立臺灣大學/生物資源暨農學院/畜產學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.02080

摘要

本試驗目的為探討固醇調節區域結合蛋白（Sterol regulatory element binding protein, SREBP）在雞隻中對於脂質新生成及膽固醇合成代謝基因的調控功能。本試驗分為兩部分進行，試驗一：將16隻7週齡肉雞隨機分成兩組，每組8隻，一組禁食24小時（禁食組），一組禁食24小時後給予任食5小時（飽食組），以即時定量聚合

關鍵字

雞隻 ； 肝臟 ； 固醇調結區域結合蛋白 ； 脂質新生成

並列摘要

To study the role of sterol regulatory element binding-proteins (SREBPs) in lipogenesis and cholesterol synthesis in the chicken, two experiments were carried out. First, 16 seven-week-old broilers were allocated into 2 groups treated with either fasted for 24 h or refed for 5 h after a 24 h-fast. The mRNA concentrations for SREBPs and other lipogenic genes in the liver were determined by quantitative real-time PCR. The hepatic mRNA relative abundance of lipogenic genes and genes in cholesterol synthesis were significantly greater (p<0.001) in the refed than the fasted broilers. Similar results were demonstrated with northern analysis technique. The data suggest that in the livers of fasted broilers, the lipogenesis and cholesterogenesis activities were inhibited. The data also demonstrated that the expression of lipogenic genes coordinate well as a group during the refed period. Second, three small interfering RNA(siRNA) oligonucleotides against the SREBP1 were designed to be used in transfecting chicken hepatocarcinoma cell line LMH. One of the 3 siRNAs effectively reduced SREBP1 mRNA concentration (p<0.01). The acetyl coenzyme A carboxylase

並列關鍵字

chicken ； liver ； sterol regulatory element binding protein ； lipogenesis

參考文獻

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