活性氧物質對吳郭魚肝臟駐留巨噬細胞與組織修復之影響

The Effect of Reactive Oxygen Species on the Tilapia Liver Resident Macrophage and Tissue Repair

吳育昇(Yu-Sheng Wu)

指導教授 ： 陳秀男

國立臺灣大學/生命科學院/漁業科學研究所/博士(2014年)

https://doi.org/10.6342/NTU.2014.02672

摘要

活性氧反應中產生之過氧化氫具有吸引肝臟駐留巨噬細胞，活化其吞噬能力的功能性存在。研究結果顯示，發炎性反應導致肝臟細胞產生大量活性氧物質，並誘導肝臟駐留巨噬細胞遷移至發炎區域且活化巨噬細胞進行清除外來物質的功能。
NAPDH Oxidase受到抑制後，組織、細胞進行傷害試驗，結果指出，組織、細胞無法有效進行活性氧物質反應，將導致組織、細胞的修復功能被抑制。本論文指出，活性氧物質具有"訊息者"的角色，生物體受到內在或外來刺激時，大量產生的活性氧物質不僅針對清除外來病原菌，亦具有活化週遭免疫細胞，如:駐留巨噬細胞，血液單核球/巨噬細胞，並且誘導受傷區塊的組織、細胞進行修復等功能。

關鍵字

巨噬細胞 ； 活性氧物質 ； 發炎 ； 修復 ； 細胞生理

並列摘要

After the injury or wound, the series of physiological response was initiated that induce the early stage of immune response moreover the late stage of wound repair process. In the series responses, inflammation is a very important role of the physiological reaction that including inflammation materials such as ROS, RNS, inflammatory substances and anti-inflammatory substances, etc., for the modulation of injury response and to avoid the invasion of pathogenic organisms . In the end stage of the inflammation reaction, the injured cell or tissue are supposed to be in the process of wound repair to recover and remodel the function and status of injured tissue. Reactive oxygen species (ROS) may act as signaling molecules in the physiology responses and the present study aims to investigate the effect of extracellular hydrogen peroxide on macrophages cellular response. The results obtained in the present study showed that the extracellular hydrogen peroxide affectively alter the membrane potential of the cell membrane and ion exchange channels in the cell membrane through intracellular NAD turnover that may lead to an intracellular calcium ion concentration alteration and subsequently induce the downstream signal activation, furthermore to realize the role of NADPH generation ROS involved in the tissue repair. We consider that from the wound to the healing process, the physiology point key to linkage of the process is still unclear. The process from inflammation to the wound healing is divided into three phase: 1. inflammation process, 2. tissue formation, and 3. tissue remodeling. The inflammation program including cell produced related factors and immune cells infiltration. We thought the inflammation factors that may be also involved in the followed healing process. But the question is “what kind of factor is the major key involved in the end of the inflammation then to initiate the healing”. We suspect that the apoptosis of immune cell may be the major key to end of inflammation and to initiate the healing.

並列關鍵字

Macrophage ； Reactive Oxygen Species ； Inflammation ； Repair ； Cellular physiology

參考文獻

1. Tacke F, Luedde T, Trautwein C: Inflammatory pathways in liver homeostasis and liver injury. Clin Rev Allergy Immunol 2009, 36(1):4-12.

2. Hotamisligil GS: Inflammation and metabolic disorders. Nature 2006, 444(7121):860-867.

3. Martinon F, Mayor A, Tschopp J: The inflammasomes: guardians of the body. Annu Rev Immunol 2009, 27:229-265.

4. Fink MP: Role of reactive oxygen and nitrogen species in acute respiratory distress syndrome. Curr Opin Crit Care 2002, 8(1):6-11.

5. Novo E, Busletta C, Bonzo LV, Povero D, Paternostro C, Mareschi K, Ferrero I, David E, Bertolani C, Caligiuri A et al: Intracellular reactive oxygen species are required for directional migration of resident and bone marrow-derived hepatic pro-fibrogenic cells. J Hepatol 2011, 54(5):964-974.