以化學修飾法與定點突變法研究無乳鏈球菌之 唾液酸合成酶內必需的半胱胺酸與精胺酸

Chemical Modification and Site-directed Mutagenesis of Cys and Arg of the Sialic Acid Synthase from Streptococcus agalactiae

陳瑞娟(Jui-Chuan Chen)

指導教授 ： 林俊宏

國立臺灣大學/生命科學院/生化科學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.01707

摘要

B型無乳鏈球菌（Group B Streptococcus agalactiae）含唾液酸的莢膜，能避免補體C3b的沉澱或巨噬細胞的吞噬，使菌體可經由垂直感染造成新生兒敗血症或腦膜炎。莢膜多醣的產生及其結構的完整性，已被報導為該菌的致病毒性因子。而唾液酸位於莢膜上寡醣單元（NeuAc-

關鍵字

唾液酸合成酶

並列摘要

Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. The production of a type-specific capsular polysaccharide (CPS) is essential to the virulence of this pathogen and enables the bacteria to evade host immue defenses. As a key component of the capsular polysaccharide, sialic acid (or N-acetylneuraminic acid) is

並列關鍵字

sialic acid synthase

參考文獻

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5. Rodriguez-Aparicio, L.B., Ferrero, M.A., and Reglero, A. (1995) N-Acetyl-D-neuraminic acid synthesis in Escherichia coli K1 occurs through condensation of N-acetyl-D-mannosamine and pyruvate. Biochem.J. 308, 501-505.