細胞於二元自組裝單層膜修飾之金表面的貼附與生長行為

Cell Proliferation on Binary Self-Assembled Monolayers Modified Gold Surfaces

黃芝潔(Chih-Chieh Huang)

指導教授 ： 薛景中

國立臺灣大學/工學院/材料科學與工程學研究所/碩士(2013年)

https://doi.org/10.6342/NTU.2013.02996

摘要

細胞貼附行為是許多細胞行為的基礎，影響細胞訊息的傳導也影響細胞個體的存活、分裂與能動性。隨著近年來生醫材料和各項生物檢測設備的發展，細胞於人造表面的貼附行為研究也愈來愈受到重視。目前被發展出來用於研究細胞與人造表面互動的系統有蛋白質覆蓋的聚苯乙烯表面、高分子薄膜、熱感性高分子、水凝膠和自組裝單層膜 (SAMs)，其中自組裝單層膜比其他系統更能精準的控制表面組成，因而能進一步控制表面性質。
為了進一步提高表面改質的變化與彈性，本研究採用兩種官能基混合修飾金基材的自組裝單層膜系統。採用的自組裝單層膜分子為6-胺基-1-己基硫醇和6-酸基1-己基硫醇，在pH 7.4的環境下兩種分子分別修飾的金表面，其表面電位差異約為200 mV。因此藉由將兩種分子以不同比例混合便能形成一系列表面電位的自組裝單層膜，並以此一系列試片探討表面電位對細胞行為的影響。
研究中採用X射線光電子光譜儀 (XPS) 量測自組裝單層膜表面的化學組成，以電動力學分析儀 (electrokinetic analyzer) 量測自組裝單層膜的表面電位。另外以光學顯微鏡、螢光顯微鏡以及電子顯微鏡觀察小鼠胚胎纖維細胞株NIH3T3在各個混合比例的自組裝單層膜上的貼附與生長行為，包含貼附量和生長量和形貌。研究發現，由於細胞表面整體帶負電性，基材表面上-NH2比例愈多者，表面電位愈正，細胞的生長情況也愈好，數量愈多，細胞形狀較健康，反之，基材表面上-NH2比例愈低者，表面電位愈負，細胞生長情況愈差，數量愈少，細胞形狀較不健康。

關鍵字

細胞貼附 ； 細胞生長 ； 自組裝單層膜 ； 混合官能基 ； 表面電位

並列摘要

Cell adhesion is central to many cell behaviors including survival, differentiation, and motility. With the recent development of biomaterials and medical instrumentation, cell behaviors on artificial biosurface have gained attention among researchers. Current tools used for researching these behaviors includes protein-coating polystyrene surface, polymer thin films, thermoresponsive polymer, hydrogel and self-assembled monolayers.
Self-assembled monolayers are known for its excellent ability to modify surface. To acquire better control of surface properties, mixed functional groups of alkanthiolates are introduced to gold surface. In this study, 6-amino-1-hexanethiol and 6-mercaptohexanoic acid are applied to gold substrate. Zeta potential of 6-amino-1-hexanethiol and 6-mercaptohexanoic acid are -187 and 6 mV, respectively, with the difference of 193 mV. Thus, a series of zeta potential are obtained through deposition of mixed alkanethiolates of different ratios on gold substrate.
Surface chemical compositions of SAMs are measured by X-ray photoelectron spectroscopy, while that of zeta potential are determined by electrokinetic analyzer. To discuss cell adherence and proliferation, NIH3T3 cells morphology and amount are investigated using optical microscope, fluorescence microscope and scanning electron microscope. The result shows that the more positive the zeta potential, the better the cells adhere and grow. On the contrary, the more negative the zeta potential, the worst the cells adhere and grow.

並列關鍵字

Cell adhesion ； cell proliferation ； self-assembled monolayers ； mixed functional groups ； surface potential ； zeta potential

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