- 學位論文
α-亞甲基-γ-五環內醯胺化合物之生物活性研究
Investigation of biological activities of α-methylene-γ-lactam compounds
摘要
我們已知α-亞甲基-γ-五環內醯胺化合物(α-methylene-γ- lactam)證實對癌症細胞有毒殺效果，在生物活性上也擁有廣泛的應用，像是消炎、抗菌、抗黴菌…等。為了瞭解此化合物能否作為腎癌細胞的毒殺藥物，我們必須進一步探討其對腎臟細胞之毒性高低。我們利用實驗室合成出來的八種α-亞甲基-γ-五環內醯胺化合物作為試驗藥品，選用腎臟可發綠螢光之基因轉殖斑馬魚Tg(wt1b:EGFP)為生物模型，藉此探討其對腎臟造成的影響，以用來評估化合物之安全性。我們將24 hpf的斑馬魚胚胎浸泡於六組不同的條件中，分別是三種濃度(1 nM、10 nM、100 nM)與兩個時間（24-36 hpf、24-72 hpf）之組合，發現腎臟的畸形率會與濃度成正比(1nM，10-100%；10nM，16.7-100%；100nM，16.7-100%)，發現在長時間浸泡下的斑馬魚胚胎，其嚴重畸形與輕微畸形的比例下降，推測可能是隨著胚胎的成熟，腎臟自我修復的能力增強所導致。存活率分析的部分，除了編號5的α-亞甲基-γ-五環內醯胺化合物在長時間的浸泡下存活率會低於80%，其餘實驗結果則皆達100%存活率，因此，α-亞甲基-γ-五環內醯胺化合物具有潛力作為腎癌細胞毒殺藥物的可行性。
並列摘要
The α-methylene-γ-lactams have known as antitumor agents and own lower cytotoxicity which compared to α-methylene-γ-lactaones, and present many biological activities, such as anti-inflammatory, antibacterial, and antifungal…etc. In order to understand these compounds may serve as antitumor drugs for kidney cancer cells, This experiment synthesized eight kindsα-methylene-γ-lactam compound by our laboratory, and used transgenic zebrafish Tg(wt1b:EGFP) as the toxicity investigation model to investigated their toxicities for normal cells. The stage of 24 hpf zebrafishes were treated six different protocols, which comprised combinations of three concentrations(1, 10, 100 nM) and two exposure methods [methods I, 24-36 hpf(hours post- fertilization); II, 24-72 hpf]. The results showed that the malformation rate of kidney is proportion to the concentration of introducing γ-lactam(1nM, 10-100%；10nM,16.7-100%；100nM，16.7-100%), the longer time soaking of embryo with α-methylene-γ-lactam decrease the severe and mild ratio of embryo’s malformation rate, this observation may cause by kidney self-repair mechanism which increased with embryo mature; All testing γ-lactams exhibit normal cell survival rate reach 100%, except the No.5 compound. Therefore, the α-methylene-γ-lactam as renal cancer medication candidate may be achieved after more studies.
參考文獻
延伸閱讀
- 陳彥豪（2012）。含胺基五苯荑衍生物之合成及其與苯胺電化學共聚合之性質研究〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2012.10695
- 李格睿（2006）。α-亞甲基-β−胺基酯進行高立體位向選擇性麥可加成反應〔碩士論文，淡江大學〕。華藝線上圖書館。https://doi.org/10.6846/TKU.2006.00934
- 洪永叁、翁健隆（2001）。α-亞甲基內酯化合物及Methylenolactocin的合成。化學，59(1)，1-10。https://doi.org/10.6623/chem.2001001
- Ko, S. (2007). The improvement in the synthesis of bioactive heterocyclic molecules [doctoral dissertation, National Taiwan Normal University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0021-0204200815531623
- 黃建峰（2016）。Investigation of Structure Activity Relationship of Phenylacetamide〔碩士論文，義守大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0074-1508201613274500