五氧化二釩在斑馬魚體內的影響以及與鎂的相互作用

釩（Vanadium , V）為存在海水中第二豐富的過渡金屬，在地殼中含量約 0.02％。它存在於岩石、砂等固體沉積物中，雖不易溶解於水，但可透過水運輸。釩化合物主要透過煤或化石燃料的燃燒存在於空氣中，並以幾種氧化態 V2O3、V2O4 和 V2O5 存在。其中五氧化二釩（V2O5）被國際癌症研究協會（IARC）分類為可能對人類致癌的物質，但現今尚未有足夠的證據證明。本研究的目的為找到 V2O5 致癌反應更相關的證據。在 V2O5 毒性的已知機制中，氧化壓力對生物系統最為關鍵。基於釩的還原，產生活性氧化物（ROS）造成氧化壓力相關的毒性。因此，本研究旨在評估 V2O5 對斑馬魚的急性影響。我們先計算 V2O5 對斑馬魚胚胎發育急性影響的致死濃度。我們試圖用硫酸鎂（MgSO4）拯救斑馬魚。為了鑑定斑馬魚中抗氧化基因的表達變化，我們使用定量 PCR（qPCR）方法，包括 5 個推定的抗氧化基因（gstp1, gclc, gpx1a, sod1, sod2），和壓力反應基因（hsp70）。我們的結果顯示 MgSO4 在 V2O5 給藥中的有益作用。在未來，經重複暴露實驗後的結果，將是決定特定釩化合物的性質和對人類健康潛在風險的關鍵。

關鍵字

釩；五氧化二釩；氧化壓力；斑馬魚

並列摘要

Vanadium (V), the second most abundant transition metal in seawater, is composed of 0.02% in the crust. The vanadium compounds are mainly present in air by combustion of coal or fossil fuel. Vanadium commonly exists in several oxidation states V2O3, V2O4, and V2O5. The International Association for Research on Cancer (IARC) classifies vanadium pentoxide (V2O5) as a substance that may be carcinogenic to humans. Since there is insufficient evidence against the carcinogenic responses to V2O5, to find more relevant evidence is the aim of this study. Among the known mechanisms of V2O5 toxicity, the oxidative stress is of particular importance for biological systems. The toxicity associated with oxidative stress is based on the reduction of vanadium, creating reactive oxygen species (ROS). Therefore, this study intends to evaluate the acute effects of V2O5 to zebrafish. We evaluate acute effects of V2O5 lethal concentration by the embryonic development. We used magnesium sulfate (MgSO4) to rescue zebrafish. To identify oxidative stress-responsive gene expression changes in zebrafish, we used quantitative PCR (qPCR) method, include five putative antioxidant genes (gstp1, gclc, gpx1a, sod1, sod2), and a stress response gene (hsp70). Our results show the beneficial action of MgSO4 upon the V2O5 administration. In the future, repeated exposures work will be crucial in figuring out how the properties of specific vanadium compounds and the potential risks on human health.

並列關鍵字

Vanadium ； Vanadium pentoxide ； Oxidative stress ； Zebrafish

參考文獻

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五氧化二釩在斑馬魚體內的影響以及與鎂的相互作用

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