以微觀角度探討白蛋白與玻尿酸在人工關節材料摩擦下之潤滑行為

人工關節摩擦時所產生的磨耗顆粒會引起人體生理反應，尤其是超高分子量聚乙烯與Co-Cr-Mo合金的磨耗顆粒更會引發一連串免疫反應，造成人工關節鬆脫而須進行人工關節再置換手術。關節液分子藉由不同的潤滑機制保護對磨表面，達到潤滑的效果，而不同關節液分子於潤滑時所扮演的功能性也不同；不同關節液分子間也會有交互作用，亦會造成不同的潤滑機制。因此我們希望能深入探討關節液成分對人工關節材料的潤滑行為，以瞭解潤滑液分子於摩擦系統中所扮演的角色。本研究利用奈米力學量測系統，以微觀的角度針對潤滑液的潤滑行為進行深入探討，期望能分析出潤滑液分子微觀的潤滑性質，藉以討論潤滑液分子巨觀的潤滑行為。從實驗結果發現單成份白蛋白濃度越高時刮痕摩擦係數越大，但單成份玻尿酸的刮痕摩擦係數並不會隨濃度升高而變化；白蛋白溶液中添加玻尿酸並無降低刮痕摩擦係數，且刮痕摩擦係數隨混合液中白蛋白濃度升高而增加，因此我們推測白蛋白會吸附於Co-Cr-Mo合金而影響摩擦係數，玻尿酸則較無吸附行為而對刮痕摩擦係數較無影響。當白蛋白因關節摩擦生熱而受熱變性時，其刮痕摩擦係數將隨受熱溫度升高而增加，且白蛋白的變性將使其吸附薄膜變形能力降低且硬度增加，由此推測白蛋白吸附薄膜之機械性質變化為摩擦係數升高的原因。未來仍須對不同關節液分子進行微觀刮痕實驗，以釐清各種潤滑液分子之潤滑機制，找出最佳潤滑效果之潤滑液成份。

關鍵字

鈷鉻鉬合金；關節液；摩擦性質；奈米壓痕

並列摘要

The wear of artificial joint materials lead to the generation of wear debris, triggering a series of immunological reactions in human body. Different lubricants in the synovial fluid play different roles in the friction system. The interaction of each components in the friction system would also influence the lubrication ability. Hence, in order to realize the roles of each lubricants, we have to investigate the influence of synovial fluid components on the lubrication behaviors of artificial joints. In this study, we investigated the lubrication behavior of synovial fluid components in microscopic view by nano-indenter. In the single component system, the results show that the friction coefficient raise with increasing albumin concentration. However, when hyaluronic acid concentration increase, the friction coefficient would not change. As adding hyaluronic acid into albumin solution, the friction coefficient would not decrease, and the friction coefficient raise with increasing albumin concentration in the mixture. We speculate that the adsorption of albumin on Co-Cr-Mo alloy would affect the friction coefficient, but hyaluronic acid wouldn’t affect the friction coefficient in microscopic view due to the inexistence of the adsorbed behavior of hyaluronic acid. The denatured albumin’s friction coefficient is higher than the nature albumin, and the adsorbed film of denature albumin is harder than nature albumin. We think that’s the reason why the friction coefficient increase as nature albumin becoming denature albumin. In the future, the lubricants still need to be studied, so that we can find out the best lubricant.

並列關鍵字

Co-Cr-Mo alloy ； Synovial Fluid ； Friction ； Nano-indentation

參考文獻

[58] 楊秉豐，奈米薄膜之微觀特性研究，博士論文，國立成功大學工程科學研究所，2010。

phosphatidylcholine as a boundary lubricant for articular cartilage.” Proceedings of phosphatidylcholine as a boundary lubricant for articular cartilage.” Proceedings of the Institution of Mechanical Engineers Part H, vol.207, 1993, pp. 59-66.

[3] Forster, H. Fisher, J. “The influence of loading time and lubricant on the friction of articular cartilage.” Proceedings of the Institution of Mechanical Engineers Part H, vol. 210, 1996, pp. 109-119.

morphological characterisatio of UHMWPE wear debris generated in vitro,”

Biomaterials, vol.22, 2001, pp. 2325-2331.

國際替代計量

以微觀角度探討白蛋白與玻尿酸在人工關節材料摩擦下之潤滑行為

全文下載

主題瀏覽