以快速原型法製作幾丁聚醣複合材料之3D支架及其對於成骨組織修復之研究

Using Rapid Prototyping System to Produce Chitosan Composite 3D Scaffolds for Bone Tissue Repair

劉易昕(I-Hsin Liu)

指導教授 ： 林忻怡

國立臺北科技大學/工程學院/化學工程研究所/碩士(2012年)

https://doi.org/10.6841/NTUT.2012.00268

摘要

快速原型系統(rapid prototyping (RP) system)可以製作出孔洞互連的多孔性支架，可使細胞均勻分布於支架中，並有利於細胞養分與代謝物運輸，刺激細胞生長、分化和形成細胞外間質(Extracellular Matrix, ECM)。幾丁聚醣可刺激骨細胞分化並促使骨骼生成，可應用於暫時性骨科補材或骨丁骨板。先前的研究少有以快速原型系統製作幾丁聚醣與其複合材料之多孔支架。本研究以快速原型技術，製作出幾丁聚醣(C)、幾丁聚醣與槴子素交聯(CG)，以及幾丁聚醣與果膠交聯(CP)之多孔性支架，並與以冷凍乾燥法製成的幾丁聚醣支架(CFD)做對照組，比較不同樣品物理性質以及生物相容性之差異，來評估材料是否適合促進骨整合。
物理測試的結果顯示CFD這組的孔隙度較其它組來的大，所以造成其降解也較其它組快。CG、CP這兩組在拉伸及壓縮的強度都較其它組來得高。而生物相容性的部分，透過掃描式電子顯微鏡觀察骨母細胞的生長型態，以DNA、鹼性磷酸酶和膠原蛋白定量來測定細胞生長與分化，並利用Von kossa鈣質染色以及鈣質定量觀察礦化程度。實驗結果顯示，C，CG，CP等組在DNA、鈣質和膠原蛋白定量的測定都較CFD組為高。
由結果得知，在生物相容性的測試上，以快速原型技術製作的多孔性支架較冷凍乾燥法來得好，而複合材料的機械強度又較純幾丁聚醣好，因此，本研究所測試之樣品中，以快速原型技術製作CG和CP多孔性支架較為適合用於促進骨整合的材料。

關鍵字

快速原型系統 ； 幾丁聚醣 ； 骨母細胞

並列摘要

Rapid prototyping (RP) systems produce porous scaffolds with interconnecting pores with evenly distributed cells. This is beneficial for nutritional and metabolic substance transportation to stimulate cell growth, differentiation, and production of extracellular matrix (ECM). Chitosan stimulates the differentiation of bone cells to promote bone growth and has application in temporary orthopedic filling materials, screws, and plates. Previous studies have neglected porous scaffolds made by RP systems with chitosan and composite materials. We have implemented RP technology to produce chitosan (C), chitosan-genipin cross-linked (CG), and chitosan-pectin cross-linked (CP) porous scaffolds. Further, we have compared them with lyophilization produced chitosan scaffolds (CFD) to identify the physical and biological compatibility characteristics differences to assess the suitability of the aforementioned materials to facilitate osseointegration.
Physical tests showed that the CFD material had the largest pore diameter when compared to other materials, which means that the degradation of CFD is comparatively faster. The CG and CP materials had the greatest elasticity, flexibility, and strength. Biocompatibility characteristics were determined through the observation of the osteoblast growth formed through a scanning electronic microscope, and growth and differentiation were obtained through the quantities of DNA, alkaline phosphatase, and collagen. Furthermore, the degree of pyritization was measured using Von Kossa calcium staining and calcium quantity. Results show that C, CG, and CP had higher measurements in DNA, calcium, and collagen when compared to CFD.
Results show that the biocompatibility of the materials made from RP techniques were better than by lyophilization and the mechanical strength of compound materials was better than pure chitosan. We have concluded that RP produced CG and CP porous scaffolds were optimal materials for the application in osseointegration.

並列關鍵字

rapid prototyping system ； chitosan ； osteoblast

參考文獻

[24] 葉志宗, 以幾丁聚醣為基質製備應用於藥物釋放之組織工程多孔性支架. 國

[1] Khor, E. and L.Y. Lim, Implantable applications of chitin and chitosan.

Biomaterials, 2003. 24(13): p. 2339-49.

[3] Kim, I.Y., et al., Chitosan and its derivatives for tissue engineering applications.

Biotechnol Adv, 2008. 26(1): p. 1-21.