本研究應用40奈米之Ag球形奈米顆粒及90奈米之SiO2球形奈米顆粒於血糖感測上,改善葡萄糖氧化酶(GOD)的活性和穩定性。將兩種奈米顆粒配合含鈷赤血鹽用來製作電流式葡萄糖感測器,將其作為電子傳媒以混入載體樹酯中。從實驗中所量測之循環伏安圖得知所添加之Ag奈米顆粒及SiO2奈米顆粒對感測電極的靈敏度提升了三倍以上,應答電流也因奈米顆粒的添加提升了兩倍。實驗中發現碳粉對樹酯比以4:10為最佳。實驗中亦指出碳粉與電子傳媒之混合比以6:4 用於碳糊電極效果最佳。實驗之偵測電位選擇於-200 mV 以避免溶液中干擾物質氧化產生電流干擾。另外,使用網狀高分子Nafion 固定GOD可製作葡萄糖感測器。考慮人體一天之內血糖濃度變化範圍以及美國食品和藥品管理協會(FDA)對血糖計容許之誤差標準15%,因此所訂之目標偵測極限我們期望至少為0.5 mM,目標線性範圍為3 ~7 mM,量測結果偵測極限為0.5mM已達成。此外,對電極之保存期限探討,本研究將電極存放於乾燥室溫下兩星期後,活性約下降40 %。
In this study, Ag spherical nanoparticles of 40 nm and 90 nm SiO2 spherical nanoparticles of glucose sensing on improving glucose oxidase (GOD) activity and stability. The two cobalt nanoparticles with the current formula used to make ferricyanide glucose sensor, the electronic media as a carrier resin in order to mix it. From the experiment measured the amount of cyclic voltammograms that Ag nanoparticles and SiO2 nanoparticles added to the sensitivity of the sensing electrodes to enhance more than tripled, adding particles to enhance the response current Yin Naimi twice. It was found that the toner resin ratio of 4:10 for the best. Experiments also pointed out that mixing ratio of carbon and electronic media to six: four carbon paste electrode for the best results. Experiments to detect potential choice to -200 mV to avoid interfering substances in solution oxidation current interference. In addition, the use of a fixed network polymer Nafion GOD glucose sensor can be produced. Consider the human body within a day of blood glucose concentration range and the American Association of Food and Drug Administration (FDA) for the standard error of 15% glucose meter allowed, so the target set by the detection limit we expect at least 0.5 mM, linear target range of 3 ~ 7 mM, measured results for 0.5mM detection limit has been reached. In addition, the shelf life of the electrodes of the discussion in this study will be deposited in the electrode dry at room temperature for two weeks, the activity decreased by about 40%.