微米粒子在藥物傳遞系統上,有保護藥物活性、標靶給藥、控制釋放速率等眾多優勢。聚乳酸與聚甘醇酸共聚物(poly (DL-lactide-co-glycolide),PLGA)為生物可分解高分子,常使用於生醫工程上,藉由乳酸與甘醇酸的莫爾比例調整,可調控高分子性質。雷帕黴素(Rapamycin)在臨床上,有抑制免疫反應與抗發炎的療效,也能抑制癌細胞有絲分裂,達到抗癌效果。在老鼠活體實驗的文獻中也指出能使神經細胞再生,促進脊神經修復。本實驗將Rapamycin包覆於PLGA微粒中,再將PLGA微粒鑲入多層蠶絲蛋白與明膠複合膜中,藉由調整蠶絲蛋白與明膠比例,控制藥物釋放速率。 以乳化蒸散法製作微粒,粒徑大小與形貌經掃描式電子顯微鏡觀察,直徑約10μm,以分光光度計(278nm)測得包藥率為43.4%,經30天累積藥物釋率放可達98.6%。若把微粒包埋在1:1ヽ2:1ヽ4:1的蠶絲蛋白與明膠混合比例複合膜中,經30天累積釋放率分別為84.9%ヽ74.7%ヽ67.4%,此包覆法能有效緩慢釋放出Rapamycin,並改善一般載體有藥物突釋的現象。
Nanoparticle has a wide range of advantages to protect active pharmaceutical ingredient, targeted medicine as well as control release rate in the drug delivery system. PLGA is biodegradable high polymer and commonly-used in biomedical tissue engineering by the mole ratio adjustment between lactic acid and glycolic acid in order to control high polymer ability. Rapamycin clinically inhibit immunological reaction and cure anti-inflammatory as well as inhibit cancer cells mitotic that will reach anti-cancer effect. This experiment will be focused on including Rapamycin into PLGA and making PLGA into multi-layer fibroin and synthesized thin films. Then, with the ratio adjustment between fibroin and synthesized thin film, we will make controlled drug release ratio. With solvent evaporation method, we analyze how to make particles and what the particle size and shape like by electron microscope with 10cm diameter. We examine the medication package of 43.4% from UV spectrometers and the accumulated drug release rate can reach at 98.61% in thirty days. With the mixed ratio of fibroin and synthesized thin films in the particles for 1:1ヽ2:1ヽ4:1, the total accumulated release rate will be 84.9%ヽ74.7%ヽ67.4% individually. This package method can release Rapamycin slowly but effectively that will improve drug burst phenomenon.