探討Antroquinonol對高脂飲食誘發肥胖大鼠之能量代謝機制

Study on energy-metabolic mechanisms of Antroquinonol in high-fat diet-induced obese rats

譚琇勻(Hsiu-Yun Tan)

指導教授 ： 陳俊榮

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2016年)

摘要

本次實驗以高脂的致肥胖飲食誘發大鼠肥胖，並觀察同時給予Antroquinonol是否可以達到預防肥胖和腹部脂肪堆積的作用，並探討可能影響的能量代謝機制。實驗分為四組：控制組、肥胖飲食組、低劑量Antroquinonol介入組 (25 mg/kg BW)及高劑量Antroquinonol介入組 (50 mg/kg BW)進行為期12週的實驗。結果發現肥胖飲食組體重與腹部脂肪重量皆顯著高於控制組，而在Antroquininol介入後則顯著降低，此外在肥胖飲食組可觀察到由腹部脂肪堆積所導致的代謝異常現象包含有：adipokines的分泌異常、肝臟脂質異位堆積、肝功能異常和肝臟分泌的促發炎激素的濃度顯著上升，然而以上現象在Antroquinonol介入後均有顯著改善的作用。進一步探討其可能機制發現Antroquinonol可透過抑制肝臟中fatty acid synthase的mRNA表現量而減少肝臟中脂質的生成作用，另外Antroquinonol也具有增加肌肉中FNDC5 (Fibronectin type III domain-containing 5)蛋白質表現量的作用，可能具有增加體內生熱作用的功能。因此，由本次的實驗結果發現Antroquinonol可藉由抑制肝臟中脂質生合成和增加體內生熱效應，達到改善經由致肥胖飲食所誘導肥胖大鼠腹部脂肪堆積和代謝異常的情形。

關鍵字

Antroquinonol ； 肥胖 ； 腹部脂肪堆積 ； 能量失衡 ； 生熱作用

並列摘要

In this study, we used obesogenic diet to observe the effects of a different dose of Antroquinonol on obesity and abdominal fat accumulation in rats. The rats were divided into four groups: control, obesity, low-does Antroquinonol (25 mg/kg BW), and high-does Antroquinonol (50 mg/kg BW). The experiment period was 12 weeks. At the end of the study, the body weight and the accumulation of abdominal fat significantly elevated in obesity group compare to the control group, and rats treated with Antroquinonol had significantly decrease in those index from obesity group. In addition, the obesity group accompanied with the metabolic dysregulation including adipokines imbalance, hepatic ectopic fat accumulation, abnormal liver function and increasing hepatic pro-inflammatory cytokines secretion. However, the intervention of Antroquinonol can significantly alleviate the obesity-induced metabolic dysregulation. To further investigate the possible mechanism, we found that Antroquinonol reduced lipogenesis in the liver by significantly inhibiting the mRNA expression of fatty acid synthase. On the other sides, the expression of thermogenesis-related protein FNDC5 (Fibronectin type III domain-containing 5) significantly increased in the high-dose intervention group. In conclusion, Antroquinonol can ameliorate diet-induced obesity and its related metabolic dysregulation in rats, through down-regulating lipogenesis and enhance thermogenesis.

並列關鍵字

Antroquinonol ； obesity ； abdominal fat accumulation ； energy imbalance ； thermogenesis

參考文獻

參考文獻

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