酸漿屬(Physalis)包括約75種,主要分佈在美洲的熱帶和溫帶地區。大多數酸漿屬已經在亞洲和美洲人的民族醫學民間傳統中使用了很長時間來治療不同的疾病,如利尿,平喘,肝炎,風濕病,瘧疾。祕魯苦蘵(Physalis peruviana)是亞熱帶地區的多年生草本植物,屬於茄科。它具有生物活性作為抗癌劑,抗肝炎,解熱劑和免疫調節劑。在先前天然物研究中祕魯苦蘵被證明含有withanolides、physalins、三萜類化合物、類固醇和生物鹼。本篇研究的重點是withasteroid化合物。它為一組多氧的C28接上麥角甾醇內酯或乳糖,是酸漿植物最具特色的成分。由於它們具有不同的化學結構和生物活性,已經廣泛研究withanolides它們的生物和藥理活性。 在生物活性測試中,祕魯苦蘵的75%MeOH層顯示出細胞毒活性。從祕魯苦蘵根的乙醇提取物中分離出七種新的withanolides(1‒7)和一種新的androstane(8)以及二十五種已知化合物(9‒33)。所有分離的化合物的結構通過光譜的方法來解釋確定,尤其是NMR分析。在細胞毒性測定中,化合物3、10‒15、23‒25、27‒28顯示出對HepG2,MDA-MB-231,A549細胞的抑製作用,IC50值範圍為0.12至17.87 μM。
The genus of Physalis comprises approximately 79 species, mainly distributed in tropical and temperate regions of America. Most species of the genus Physalis have been used for a long time in the ethnomedical folk traditions of Asian and American to treat different illnesses, such as malaria, hepatitis, dermatitis, and liver disorders. P. peruviana L. is a herbaceous, semi-shrub, upright perennial plant in subtropical zones and belongs to the family Solanaceae. In previous natural product studies, P. peruviana was proved to contain withanolides, physalins, triterpenoids, steroids, and alkaloids. This study focuses on withanolides, a group of polyoxygenated C28-ergostane lactones or lactols, are the most characteristic constituents of Physalis plants. Due to their diverse chemical structures and bioactivities, withanolides have been study extensively for their biological and pharmacological activities. In biological activities tests, the 75% MeOH layer of P. peruviana showed cytotoxic activity. Seven new withanolides (1‒7) and one new androstane compound (8) together with twenty-five known compounds (9‒33) were isolated from the ethanolic extract of P. peruviana roots. The structures of all isolated compounds were determined by the interpretation of spectroscopic methods, especially NMR analyses. In cytotoxic assays, compounds 3、10‒15、23‒25、27‒28 showed inhibition effect against HepG2, MDA-MB-231, A549 cells with IC50 values ranging from 0.12 to 17.87 μM.