馬利筋化學成份及生物活性之研究

Studies on the Chemical Constituents and Cytotoxicity of Asclepias curassavica

黃筱筑(Hsiao-Chu Huang)

指導教授 ： 吳永昌

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2004年)

摘要

摘 要
馬利筋 (Asclepias curassavica) 屬於蘿摩科 (Asclepiadaceae) 植物，主要分布地區為熱帶以及亞熱帶。在台灣民間的使用上為混合本植物與其他民間常用之抗癌中草藥一起服用，以為治療癌症之用。但其療效及其毒性的評估尚未確認。
從馬利筋分離到二十八個化合物，包括十一個doubly linked cardenolide glycosides 類化合物: calotropin (1)，16??-acetoxycalotropin (2)，15??-hydroxycalotropin (3)，16??-acetoxycalactin (4)，calactin (5)，15??-hydroxycalactin (6)，asclepin (7)，16??-acetoxyasclepin (8)，16??-hydroxyasclepin (9)，uscharidin (10)，uscharin (11)，另外尚有uzarigenin (12)，afrogenin (13)，??-amyrin (14)，3-O-acetyloleanolic acid (15)，MR41-33-71 (16) ，20,21-dihydroxypregna-4,6-diene-3-one (17) ，??-sitosterol and stigmasterol (18 and 19)，??-sitosteryl-??-D-glucoside (20)， linoleic acid (21)，phytol (22)，2-methoxy-3-hydroxylbenzoic acid (23)，(-)-syringaresinol (24)，MR41-47-3 (25)，MR41-19-7 (26)，MR41-17-22 (27)及 MR41-17-24 (28)。其中化合物16為新化合物，而化合物24、25需再確定其立體結構。在生物活性方面，化合物12、14及22 對不同的細胞株顯現抗癌活性。所分離得到之已知化合物結構皆經與文獻資料比對後，確認其結構。

關鍵字

馬利筋 ； 尖尾鳳 ； 蘿藦科 ； 生物活性

並列摘要

Abstract
Asclepias curassavica, known as a milkweed plant, is a good source of cardiac glycosides. It distribute in tropic and subtropic area in the world. In Taiwan, it was used as a cancer drug, but its toxic and pharmcologic activitives were not clearly. To solve these problems and develop new drugs, a chemical study about Asclepias curassavica was carried out.
There are 28 compounds were isolated from Asclepias curassavica, including 11 doubly linked cardenolide glycosides, including calotropin (1), 16??-acetoxycalotropin (2), 15??-hydroxycalotropin (3), 16??-acetoxycalactin (4), calactin (5), 15??-hydroxycalactin (6), asclepin (7), 16??-acetoxyasclepin (8), 16??-hydroxyasclepin (9), uscharidin (10), uscharin (11), along with uzarigenin (12), afrogenin (13), ??-amyrin (14), 3-O-acetyloleanolic acid (15), MR41-33-71 (16), 20,21-dihydroxypregna-4,6- diene-3-one (17), ??-sitosterol and stigmasterol (18 and 19), ??-sitosteryl-??-D-glucoside (20), linoleic acid (21), phytol (22), 2-methoxy-3-hydroxylbenzoic acid (23), and (-)-syringaresinol (24), MR41-47-3 (25), MR41-19-7 (26), MR41-17-22 (27) and MR41-17-24 (28). Among them, compound 16 is supposed to be a new compound, and stereo structures of 25, 26 need to be assigned. In the other hand, compounds 12, 14 and 22 showed cytotoxic activities against different cancer cell lines. The structures of all isolated compounds were identified by their spectral data and compared with the literature data.

並列關鍵字

calotropin cardenolide gly ； Asclepias curassavica

參考文獻

第七章 參考文獻

1. Huang S.-F.； Huang T.-C. “In Asclepiadaceae”, Flora of Taiwan 1996, 4; 591-593.

2. 醫藥博覽，表含強心苷的中藥科屬與常用藥舉例http://www.100md.com/Html/TCM/Chemistry/T136.htm

3. Kazuhiro K.； Carolyn B.；Sunil K. T.； Geofferey A. C.； Norman R. F. “Potential anticancer agents V. cardiac glyosides of Asclepias albicans (Asclepiadaceae)”, Chem. Pharm. Bull. 1980, 28, 401-405.

4. http://www.changjaya-abadi.com/medicine-22.htm