鳶烏賊(Symplectoteuthis oualaniensis)是一個會發藍光的螢光烏賊,牠的螢光 蛋白叫做symplectin; 而去氫腔腸螢光素(DCL)是symplectin 的發光基質,為一個 天然的發色團。之前的研究發現合成的發色團2,4-二氟-去氫腔腸螢光素 (2,4-diF-DCL)可發出比天然的發色團(DCL)高兩倍的生物螢光量。所以我們推測 當symplectin 在pH 6 時(孵育期incubation),2,4-二氟-去氫腔腸螢光素 (2,4-diF-DCL)與天然發色團去氫腔腸螢光素(DCL)交換。 為了證明發色團的交換,各種八號位置改變成甲氧苯基(methoxyphenyl)或噻 吩(thiophene)的二氟-去氫腔腸螢光素的類似物被合成出來。這些類似物在八號位 置多增加一個共振系統,所以其紫外光-可見光吸收、螢光發射、化學發光和生物 發光的波長產生紅位移。在本篇研究中,二氟-去氫腔腸螢光素的類似物與其他可 能會在symplectin 生物發光過程中產生的物質的特徵性質被詳細檢驗。有這些合 成物質的資料,在symplectin 內的發色團交換可以被追蹤並估算出交換的比例。
A luminous squid, Symplectoteuthis oualaniensis, emits blue light, and its photoprotein has been named as symplectin. The luminous substrate of symplectin is dehydrocoelenterazine (DCL) as natural chromophore. A synthetic chromophore, 2,4-difluoro-dehydrocoelenterazine (2,4-diF-DCL), was found to emits 2 times bioluminescence light amount comparing to natural form chromophore (DCL). The exchange of chromophore between 2,4-diF-DCL and DCL in symplectin might happen during incubation at pH 6. To prove the exchange of the chromophores, several diF-DCL analogs with modification at 8-position (methoxyphenyl and thiophene) were synthesized. These analogs have one more conjugated system at the 8-position resulting in red-shift in UV-Vis absorbance, fluorescence emission, chemiluminescence and bioluminescence wavelength. In this study, the characteristics of DCL analogs and other compounds which are producible in symplectin luminescence process were examined. With these data from authentic samples, chromophore exchange in symplectin could be monitored and percentage of exchange could be estimated.
為了持續優化網站功能與使用者體驗,本網站將Cookies分析技術用於網站營運、分析和個人化服務之目的。
若您繼續瀏覽本網站,即表示您同意本網站使用Cookies。