- 學位論文
以毛細電泳進行防腐劑分析方法之研究
Study on the analyses of Preservatives by Capillary Electrophoresis
摘要
防腐劑可以抑制細菌或黴菌滋生，所以被廣泛的應用於食品、保養品及其他方面的保存。但防腐劑的使用對我們的健康是有害的。所以防腐劑的使用，可以說是必要之惡。 而在一般防腐劑分析中，常遇到對羥苯甲酸甲酯、對羥苯甲酸乙酯、對羥苯甲酸丙酯及對羥苯甲酸丁酯，此類防腐劑具有相似的分子結構及紫外光/可見光吸收，故增加分析的難度。而本研究是利用毛細管電泳為分離工具，以毛細管微胞電層析(micellar electrokinetic capillary chromatography，MEKC)及毛細管微乳化電層析(microemulsion electrokinetic capillary chromatography，MEEKC )的方式，對一般市面商品常添加之防腐劑，包含對羥苯甲酸甲酯、對羥苯甲酸乙酯、對羥苯甲酸丙酯、對羥苯甲酸丁酯等九種防腐劑，進行分析測試。並探討溫度、pH值及界面活性劑濃度對分離的影響。由實驗結果顯示，在以微胞電層析的形式進行測試時，以不同濃度的界面活性劑成功分離九種防腐劑。在以微乳化電層析進行測試時，利用溫度的變化成功的分離九種防腐劑，並分別成功應用於實際樣品的檢測上。
並列摘要
Preservatives can restrict to germ growing which are commonly added to various foods, pharmaceuticals and cosmetics in order to prolong products’ shelf life. However, preservatives were harmful to health especially when consume in large quantities. Therefore , the development of analytical method detecting preservatives is need . In the study , two methods for analyzing nine preservatives were developed ; micellar electrokinetic chromatography (MEKC), and microemulsion electrokinetic chromatography separately. The effect of temperature, buffer pH, and concentration of surfactant on separation were examined. In MEKC, the separation of preservatives improved markedly by changing the sodium dodecyl sulfate concentration. In (microemulsion electrokinetic chromatography)MEEKC, the separation of preservatives improved by changing temperature. An optimum MEKC method for separation of the nine was obtained within 9.0 min with a running buffer of pH 9.0 containing 20mM SDS at 25℃.A separation with baseline resolution was obtained within 16 min using a microemulsion of pH 9.5 which composed of SDS, 1-butanol, and octane at 34℃.Finally, the developed MEKC and MEEKC methods determined successfully preservatives in various cosmetic and pharmaceutical products.
參考文獻
延伸閱讀
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