電流式葡萄糖與尿酸感測器之研製

Study and fabrication of the amperometric glucose and uric acid sensors

鄭美玲(Mei-Lin Cheng)

指導教授 ： 熊慎幹

中原大學/工學院/醫學工程研究所/碩士(2004年)

https://doi.org/10.6840/cycu200400306

摘要

本論文係電流式感測器,主要目的係發展一個電流式葡萄糖與尿酸生物感測器。實驗架構係利用聚丙醯胺分別將葡萄糖酵素(Glucose oxidase)、尿酸酵素(Uricase)和電子傳遞媒介物(Ferrocenecarboxylic acid )固定於工作電極上。此研究主要係偵測酵素於外加電壓下,於電極上所產生之還原電流反應。此電極反應係利用酵素與尿酸或葡萄糖產生過氧化氫之反應,且利用循環伏安儀分析其特性,利用外加200mV電壓於參考電極,測量來自於酵素反應之還原電流。本研究中尿酸感測器量測濃度範圍為2.5-20 mg/dl,反應時間為65秒,線性部分之感測度為5.17uAcm-2(mg/dl)-1。而葡萄糖感測器量測濃度範圍為25-400 mg/dl,反應時間為65秒,線性部分之感測度為0.94uAcm-2(mg/dl)-1。

關鍵字

葡萄糖酵素 ； 尿酸酵素 ； 電流式感測器 ； 碳電極

並列摘要

In this study, amperometric sensors for glucose and uric acid have been developed. It is constructed by immobilizing glucose oxidase, uricase and mediator (ferrocenecarboxylic acid) on the working electrode via polyacrylaminde. The response of the electrode was based on the enzymatic reduction of hydrogen peroxide in the presence of uric acid and glucose. Cyclic voltammetry was used to characterize the perselective characteristic of the enzyme electrode. The assay was based on the measurement of a reduction current resulting from the enzymatic reaction at the enzyme electrode holding at 200mV (v.s. Ag /AgCl). The linear detection range of uric acid is shown between 2.5 mg/dl and 20 mg/dl. The sensor has a response time of 65 seconds and sensitivity is 5.17uAcm-2(mg/dl)-1 and the linear detection range of glucose is shown between 25 mg/dl and 400 mg/dl. The sensor has a response time of 65 seconds and sensitivity is 0.94uAcm-2(mg/dl)-1

並列關鍵字

Glucose oxidase ； Amperometric sensor ； Uricase ； Carbon

參考文獻

[25]徐俊傑, “應用版式電極製作可攜帶式快速尿酸感測系統”,中原大學醫學工程學系, 碩士學位論文, 2003.

[4]T. Tatsuma, T. Watanable, “Oxidase/peroxidase bilayer-modified electrodes as sensor for lactate, pyruvate, cholesterol and uric acid”, Anal Chim Acta, vol.242, pp.85-89, 1991.

[5]Shaolin. M.,Jinqing. K.,Jianbing. Z. ,“Bioelectrochemical response of the polyaniline uricase electrode”, Electroanal Chem, vol.334, pp.121-132,1992.

[6]M. T. Gilmartin, J. P. Hart, “Sensing with chemically and biologically modified carbon electrodes : a review”, Analyst, vol.120, pp. 1029 -1045, 1995.

[7]O. Eleke, D. Moscone,K. Venema, J. Korf, “Bi-enzyme reactor for electrochemical detection of low concentration of uric acid and glucose”Clin Chim Acta, vol.239, pp.153-165, 1995.