藍光對於人類疤痕纖維母細胞之影響

The Effect of Blue Light on Human Scar Fibroblasts

蔡杰麟(Jay-Lin Tsai)

指導教授 ： 謝瑞香

中原大學/工學院/醫學工程研究所/碩士(2007年)

https://doi.org/10.6840/cycu200700142

摘要

在本研究中利用發光二極體(light-emitting diode, LED)陣列組成之藍光(波長467nm)，探討藍光光刺激對於人類包皮纖維母細胞(human foreskin fibroblast)、人類正常表皮纖維母細胞(human normal dermal fibroblast)及人類肥厚疤痕纖母細胞(human hypertrophic scar fibroblast)等三種細胞的生長之影響。
利用藍光刺激上述三種細胞，皆能產生抑制生長的效果。當光刺激功率密度10 mW/cm2、能量密度固定為4及8 J/cm2時，藍光抑制三種細胞生長的效果皆在10%以內。當功率密度提升至17 mW/cm2，總能量固定為4及8 J/cm2時，藍光抑制細胞生長的效果分別為：人類包皮纖維母細胞：5%、人類正常表皮纖維母細胞：20%及人類肥厚疤痕纖維母細胞：15%。當功率密度17 mW/cm2在人類正常表皮纖維母細胞及人類肥厚疤痕纖維母細胞的結果可看到顯著差異。
就細胞之倍增時間(doubling time)方面來看，給予三種細胞藍光光刺激後，可以延長細胞之倍增時間。功率密度10 mW/cm2，總能量固定為4及8 J/cm2時，細胞倍增時間延長之倍數為：人類包皮纖維母細胞：1.01~1.03倍、人類正常表皮纖維母細胞：1.01~1.03倍及人類肥厚疤痕纖維母細胞：1.02~1.05倍。功率密度17 mW/cm2，總能量固定為4及8 J/cm2時，細胞倍增時間延長之倍數為：人類包皮纖維母細胞：1.03倍、人類正常表皮纖維母細胞：1.19~1.22倍及人類肥厚疤痕纖維母細胞：1.08~1.12倍。當增加藍光光刺激細胞的次數，同一功率及能量密度下分為單次及多次刺激，結果發現人類包皮纖維母細胞及人類正常表皮纖維母細胞中，光刺激次數與抑制效果大小無關。
利用AnnexinV /PI染色，我們可以觀察經過藍光光刺激後，是否有凋亡現象產生。結果發現刺激組沒有細胞凋亡的現象出現，因此可以確定藍光抑制細胞生長的機制與細胞凋亡無關。

就膠原蛋白分泌量來做比較，結果發現經由藍光光刺激後，照光組別膠原蛋白分泌量在第48小時較控制組多，而在第96小時發現膠原蛋白分泌量則有受到抑制的情況出現。

由上述結果可得知：藍光光刺激可以讓三種細胞之倍增時間拉長，導致細胞生長速率下降而造成抑制生長現象。

本研究結果發現，經由藍光光刺激可以抑制肥厚疤痕纖維母細胞的生長速率。

關鍵字

肥厚疤痕纖維母細胞 ； 傷口癒合 ； 發光二極體

並列摘要

The study to be described below is to evaluate the effects of blue light (467 nm) emitted by a LED array on cell growth of various fibroblasts including human foreskin (FF), normal dermal (NF) and hypertrophic scar (SF).

These results implied that blue light could inhibit the proliferation of these three types of fibroblasts. Exposure to 4 J/cm2 or 8 J/cm2 of blue light(10 mW/cm2) suppressed the number of three cells described above less 10% over no-light control. When we add the power density of blue light up to 17 mW/cm2, the blue light inhibit the number of FF, NF, and SF by 5%, 20% and 15%, vs. no-light controls. The inhibition of blue light(17 mW/cm2) on NF and SF are significant.

After blue light exposure, the doubling times increased slightly. When the power density of blue light is 10 mW/cm2, the prolongation of doubling time of FF, NF, and SF by 1.01 to 1.03, 1.01 to 1.03 and 1.02 to 1.05, vs. no-light controls. When the power density of blue light is 17 mW/cm2, the prolongation of doubling time of FF, NF, and SF by 1.03, 1.19 to 1.22 and 1.08 to 1.12, vs. no-light controls. There is no significant change in the proliferation of FF and NF, after one or three times of irradiation with blue light.

We used Annexin V/PI stain to detect the apoptosis of fibroblasts after blue light exposure. We found nothing about cells apoptosis, and thought the factor of blue light inhibit proliferation of fibroblasts has no relation to apoptosis.

The secretion of collagen of fibroblasts exposed to blue light is more than no-light controls after 24 hours of exposure, but became less after 72 hours of exposure.

This study concludes that the proliferation of fibroblasts tends to decrease slightly associated with the irradiation of blue light.

並列關鍵字

hypertrophic scar fibroblast ； wound healing ； LED

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